Abstract
We put forward an impedometric protein-based biosensor platform suitable for point-of-care diagnostics. A hand-held scale impedance reader system is described for the detection of corresponding physiochemical changes as the immobilized proteins bind to the analyte molecules in the proximity of the microfabricated electrodes. Specifically, we study the viability of this approach for glucose biosensing purposes using genetically engineered glucokinase as receptor proteins. The proposed reagent-less biosensor offers a high sensitivity of 0.5 mM glucose concentration level in the physiologically relevant range of 0.5 mM to 7.5 mM with less than 10 s response time.
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Acknowledgment
Authors would like to acknowledge support from NSERC Canada, FQRNT, and ReSMiQ.
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An erratum to this article can be found at http://dx.doi.org/10.1007/s10544-010-9462-x
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Ghafar-Zadeh, E., Chowdhury, S.F., Aliakbar, A. et al. Handheld impedance biosensor system using engineered proteinaceous receptors. Biomed Microdevices 12, 967–975 (2010). https://doi.org/10.1007/s10544-010-9451-0
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DOI: https://doi.org/10.1007/s10544-010-9451-0