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Effect of Lactoferrin on the Ferroxidase Activity of Ceruloplasmin

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Abstract

The effects of various forms of lactoferrin (Lf) interacting with ceruloplasmin (Cp, ferro-O2-oxidoreductase, EC 1.16.3.1) on oxidase activity of the latter were studied. Comparing the incorporation of Fe3+ oxidized by Cp into Lf and serum transferrin (Tf) showed that at pH 5.5 apo-Lf binds the oxidized iron seven times and at pH 7.4 four times faster than apo-Tf under the same conditions. Apo-Lf increased the oxidation rate of Fe2+ by Cp 1.25 times when Cp/Lf ratio was 1 : 1. Lf saturated with Fe3+ or Cu2+ increased the oxidation rate of iron 1.6 and 2 times when Cp to holo Lf ratios were 1 : 1 and 1 : 2, respectively. Upon adding to Cp the excess amounts of apo-Lf (Cp/apo-Lf < 1 : 1) or of holo Lf (Cp/holo-Lf < 1 : 2) the oxidation rate of iron no longer changed. Complex Cp-Lf demonstrating ferroxidase activity was discovered in breast milk.

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Abbreviations

Cp:

ceruloplasmin

Lf:

lactoferrin

Tf:

transferrin

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Authors and Affiliations

  1. Institute for Experimental Medicine, Russian Academy of Medical Sciences, ul. Akademika Pavlova 12, 197376, St. Petersburg, Russia

    A. V. Sokolov, M. O. Pulina, E. T. Zakharova, M. M. Shavlovski & V. B. Vasilyev

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  1. A. V. Sokolov
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  2. M. O. Pulina
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  3. E. T. Zakharova
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  4. M. M. Shavlovski
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  5. V. B. Vasilyev
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Correspondence to A. V. Sokolov.

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__________

Translated from Biokhimiya, Vol. 70, No. 9, 2005, pp. 1231–1236.

Original Russian Text Copyright © 2005 by Sokolov, Pulina, Zakharova, Shavlovski, Vasilyev.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM04-271, February 13, 2005.

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Sokolov, A.V., Pulina, M.O., Zakharova, E.T. et al. Effect of Lactoferrin on the Ferroxidase Activity of Ceruloplasmin. Biochemistry (Moscow) 70, 1015–1019 (2005). https://doi.org/10.1007/s10541-005-0218-9

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  • DOI: https://doi.org/10.1007/s10541-005-0218-9

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