Abstract
A low molecular weight isoform of hyaluronidase (NNH2) has been isolated from Indian cobra (Naja naja) venom by successive chromatography on Sephadex G-75 and CM-Sephadex C-25 columns. The apparent molecular weight determined by SDS-PAGE is 52 kD, and the pI value is 9.7. NNH2 is an endoglycosidase and exhibits in vitro absolute specificity for hyaluronan; it also hydrolyzed hyaluronan in human skin sections. NNH2 is nontoxic, but it indirectly potentiates the hemorrhagic activity of hemorrhagic complex-I. Curcumin, indomethacin, and tannic acid inhibited dose dependently the degradation of hyaluronan by NNH2.
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Translated from Biokhimiya, Vol. 70, No. 6, 2005, pp. 855–860.
Original Russian Text Copyright © 2005 by Girish,, Kemparaju.
Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM04-224, October 24, 2004.
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Girish, K.S., Kemparaju, K. A Low Molecular Weight Isoform of Hyaluronidase: Purification from Indian Cobra (Naja naja) Venom and Partial Characterization. Biochemistry (Moscow) 70, 708–712 (2005). https://doi.org/10.1007/s10541-005-0172-6
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DOI: https://doi.org/10.1007/s10541-005-0172-6