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A new alkalitolerant Yarrowia lipolytica yeast strain is a promising model for dissecting properties and regulation of Na+-dependent phosphate transport systems

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Abstract

A newly isolated osmo-, salt-, and alkalitolerant Yarrowia lipolytica yeast strain is distinguished from other yeast species by its capacity to grow vigorously at alkaline pH values (9.7), which makes it a promising model organism for studying Na+-dependent phosphate transport systems in yeasts. Phosphate uptake by Y. lipolytica cells grown at pH 9.7 was mediated by several kinetically discrete Na+-dependent systems specifically activated by Na+. One of these, a low-affinity transporter, operated at high concentrations of extracellular phosphate. The other two, high-affinity systems, maximally active in phosphate-starved cells, were repressed or derepressed depending on the prevailing extracellular phosphate concentration and pH value. The contribution of Na+/Pi-cotransport systems to the total cellular phosphate uptake progressively increased with increasing pH, reaching its maximum at pH ≥ 9.

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Abbreviations

CAPS:

3-[cyclohexylamino]-1-propanesulfonic acid

CCCP:

carbonyl cyanide m-chlorophenylhydrazone

DiOC6(3):

3,3’-dihexyloxacarbocyanine iodide

HPi:

high phosphate medium

LPi:

low phosphate medium

ER:

endoplasmic reticulum

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Authors and Affiliations

  1. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr. 33, 119071, Moscow, Russia

    R. A. Zvyagilskaya

  2. Department of Biochemistry and Biophysics, Stockholm University, S-106 91, Stockholm

    B. L. Persson

  3. Department of Chemistry and Biomedical Sciences, Kalmar University, S-391 82, Kalmar, Sweden

    B. L. Persson

Authors
  1. R. A. Zvyagilskaya
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  2. B. L. Persson
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Correspondence to R. A. Zvyagilskaya.

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Translated from Biokhimiya, Vol. 69, No. 11, 2004, pp. 1607–1615.

Original Russian Text Copyright © 2004 by Zvyagilskaya, Persson.

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Zvyagilskaya, R.A., Persson, B.L. A new alkalitolerant Yarrowia lipolytica yeast strain is a promising model for dissecting properties and regulation of Na+-dependent phosphate transport systems. Biochemistry (Moscow) 69, 1310–1317 (2004). https://doi.org/10.1007/s10541-005-0016-4

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