Skip to main content
SpringerLink
Log in

An Improved Culture System of Mouse Peripheral Blood Lymphocytes for Analysis of Radiation-Induced Chromosome Aberrations

  • Published:
Bioscience Reports

Abstract

There is an incentive to develop a culture system of mouse peripheral blood lymphocytes (PBLs) to serve as models for studying genotoxic effects in humans exposed to mutagens, including ionizing radiation. However, many past approaches have been laborious, complex and only partly reproducible. In the present study, we established an improved culture system of mouse PBLs by removing blood and/or plasma, which was found to inhibit in vitro mitotic stimulation or proceeding cell cycles of lymphocytes. We compared the reactions of isolated PBLs to mitogens between the classical method and the present improved one. Then, we applied this method to the cytogenetic analysis using chemically induced premature chromosome condensation (PCC) as well as the conventional analysis, and demonstrated that the frequency of excess fragments observed in PCC cells might be useful to quantify the radiation-induced damages on chromosomes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. G. L. Erexson J. L. Wilmer A. D. Kligerman (1983) ArticleTitleAnalyses of sister-chromatid exchange and cell-cycle kinetics in mouse T-and B-lymphocytes from peripheral blood cultures Mutat. Res. 109 271–281 Occurrence Handle6601771

    PubMed  Google Scholar 

  2. K. L. Triman M. T. Davisson T. H. Roderick (1975) ArticleTitleA method for preparing chromosomes form peripheral blood in the mouse Cytogenet. Cell Genet. 15 166–176 Occurrence Handle1104276

    PubMed  Google Scholar 

  3. P. R. Barren S. M. Morri H. Schol J. B. Bishop (1982) ArticleTitleA method for preparing chromosomes from peripheral blood of the mouse Mutat. Res. 104 159–163 Occurrence Handle10.1016/0165-7992(82)90138-5 Occurrence Handle7043250

    Article  PubMed  Google Scholar 

  4. G. L. Erexson A. D. Kligerman (1987) ArticleTitleA modified mouse peripheral blood lymphocyte culture system for cytogenetic analysis Environ. Mol. Mutagen. 10 377–386 Occurrence Handle3315657

    PubMed  Google Scholar 

  5. R. Kanda K. Eguchi-Kasai I. Hayata (1999) ArticleTitlePhosphatase inhibitors and premature chromosome condensation in human peripheral lymphocytes at different cell-cycle phases Somat. Cell. Mol. Genet. 25 1–8 Occurrence Handle10.1023/B:SCAM.0000007135.12486.e3 Occurrence Handle10925699

    Article  PubMed  Google Scholar 

  6. K. Koprowski P. Mocarelli T. J. Wiktor (1972) ArticleTitleAntibody response in vitro to an animal virus: production of radies virus neutralizing antibodies by mouse cells in culture Proc. Natl. Acad. Sci. USA 69 2433–2436 Occurrence Handle4341695

    PubMed  Google Scholar 

  7. I. Hayata H. Tabuchi A. Furukawa N. Okabe M. Yamamoto K. Sato (1992) ArticleTitleRobot system for preparing lymphocyte chromosome J. Radiat. Res. 33 IssueIDSuppl 231–241 Occurrence Handle1507173

    PubMed  Google Scholar 

  8. Y. Matsuda Y.N. Harada S. Natsuume-Sakai K. Lee T. Shiomi V. M. Charman (1992) ArticleTitleLocation of the mouse complement factor H gene (cfh) by FISH analysis and replication R-banding Cytogenet. Cell Genet. 61 282–285 Occurrence Handle1486806

    PubMed  Google Scholar 

  9. R. Kanda T. Jiang I. Hayata S. Kobayashi (1994) ArticleTitleEffects of colcemid concentration on chromosome aberration analysis in human lymphocytes J. Radiat. Res. 35 41–47 Occurrence Handle8057270

    PubMed  Google Scholar 

  10. K. Eguchi-Kasai H. Itsukaichi M. Murakami T. Kanai K. Shimizu K. Sato (1996) ArticleTitleEffect of exposure of carbon ion beams on cell cycle progression J. Radiat. Res. 37 309

    Google Scholar 

  11. M. D. Spruill M. J. Ramsey R. R. Swiger J. Nath J. D. Tucker (1996) ArticleTitleThe persistence of aberrations in mice induced by gamma radiation as measured by chromosome painting Mutat. Res. 356 135–145 Occurrence Handle8841478

    PubMed  Google Scholar 

  12. J. D. Tucker K. J. Sorensen C. S. Shu D. O. Nelson M. J. Ramsey S. Urlando J. A. Heddle (1998) ArticleTitleThe accumulation of chromosome aberrations and Dlb-1 mutations in mice with highly fractionated exposure to gamma radiation Mutat. Res. 400 321–335 Occurrence Handle9685691

    PubMed  Google Scholar 

  13. Takahashi, K. (1979). Mouse. In: Laboratory Animal Libraries, Ser. 2, (K. Takahashi, and T. Nobunaga, eds.), Soft Science, Tokyo, pp 203–237

  14. M. Norimatsu T. Ono A. Aoki K. Ohishi Y. Tamura (1995) ArticleTitleIn-vivo induction of apoptosis in murine lymphocytes by bacterial lipopolysaccharides J. Med. Microbiol. 43 251–257 Occurrence Handle7562985

    PubMed  Google Scholar 

  15. International Atomic Energy Agency (IAEA) (2001) Cytogenetic Analysis for Radiation Dose Assessment: a Manual, Technical Reports Series No 405, Vienna

  16. P. Boer ParticleDe P. P. W. Buu Particlevan R. Beek Particlevan F. A. Hoeven Particlevan der A. T. Natarajan (1977) ArticleTitleChromosomal radiosensitivity and karyotype in mice using cultured peripheral blood lymphocytes, and comparison with this system in man Mutat. Res. 42 379–394 Occurrence Handle857154

    PubMed  Google Scholar 

  17. R. Kanda I. Hayata D. C. Lloyd (1999) ArticleTitleEasy biodosimetry for high-dose radiation exposures using drug-induced prematurely condensed chromosomes Int. J. Radiat. Biol. 75 441–446 Occurrence Handle10.1080/095530099140366 Occurrence Handle10331849

    Article  PubMed  Google Scholar 

  18. M. Durante Y. Furusawa E. Gotoh (1998) ArticleTitleA simple method for simultaneous interphase-metaphase chromosome analysis in biodosimetry Int. J. Radiat. Biol. 74 457–462 Occurrence Handle10.1080/095530098141320 Occurrence Handle9798956

    Article  PubMed  Google Scholar 

  19. R. Kanda K. Eguchi-Kasai H. Itsukaichi M. Mori I. Hayata (1999) ArticleTitleChemically Induced Premature Chromosome Condensation in Human Fibroblast Cell Lines: Fundamental Study for Applications to the Biodosimetry of Local Exposure Somat. Cell Mol. Genet. 25 317–325 Occurrence Handle10.1023/A:1019968432644 Occurrence Handle15328910

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Radiation Hazards Research Group, National Institute of Radiological Sciences, 4-9-1, Anagawa, 263-8555, Inage-ku, Chiba, Japan

    Reiko Kanda, Yi Shang, Satsuki Tsuji, Kiyomi Eguchi-Kasai & Isamu Hayata

Authors
  1. Reiko Kanda
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yi Shang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Satsuki Tsuji
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kiyomi Eguchi-Kasai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Isamu Hayata
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Reiko Kanda.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kanda, R., Shang, Y., Tsuji, S. et al. An Improved Culture System of Mouse Peripheral Blood Lymphocytes for Analysis of Radiation-Induced Chromosome Aberrations. Biosci Rep 24, 641–650 (2004). https://doi.org/10.1007/s10540-005-2798-4

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10540-005-2798-4

Keywords

Search

Navigation