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Molecular cloning and expression analyses of FaFT, FaTFL, and FaAP1 genes in cultivated strawberry: their correlation to flower bud formation

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

In this study, we cloned flowering-related genes FLOWERING LOCUS T (FT) and TERMINAL FLOWER1 (TFL1) from domesticated octaploid strawberries (Fragaria × ananassa) and analyzed their expression patterns in cultivars Tochiotome and Akihime. The floral meristem generation was induced under the short day and low temperature (SDLT), but not under the long day and high temperature (LDHT). We found that FaFT1, which is an orthologue of the Arabidopsis floral activator FT, was highly expressed in leaves under LDHT but not expressed in leaves under SDLT. On the other hand, the expression of FaTFL2, which belongs to the TFL1 family of flowering repressing genes, decreased in crowns (stem tissue including meristem) under SDLT. These results suggest that FaTFL2, as opposed to FvTFL1 in wild diploid strawberry Fragaria vesca, is related to flowering of the cultivated strawberry. Moreover, the FaTFL2 expression might be regulated by temperature rather than by photoperiod. We demonstrated that a reduction of the FaTFL2 expression is a key signal for flowering in domesticated strawberries.

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Abbreviations

AP1:

APETALA1

CO:

CONSTANCS

DTT:

dithiothreitol

EGTA:

ethyleneglycoltetraacetic acid

FD:

FLOWERING LOCUS D

FT:

FLOWERING LOCUS T

LDHT:

long day and high temperature

LDLT:

long day and low temperature

MSI1:

multicopy suppressor of IRA1

PEBP:

phosphatidylethanolamine-binding protein

PVP:

polyvinylpyrrolidone

RT- qPCR:

real time quantitative polymerase chain reaction

SDHT:

short day and high temperature

SDLT:

short day and low temperature

SDS:

sodium dodecyl sulfate

TFL1:

TERMINAL FLOWER1

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Authors and Affiliations

  1. Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601, Japan

    R. Nakajima, S. Otagaki, K. Shiratake & S. Matsumoto

  2. College of Bioscience and Biotechnology, Chubu University, Kasugai, 487-8501, Japan

    K. Yamada

Authors
  1. R. Nakajima
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  2. S. Otagaki
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  3. K. Yamada
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  4. K. Shiratake
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  5. S. Matsumoto
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Correspondence to S. Matsumoto.

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Acknowledgements: We are indebted to Mrs. S. Niwa and K. Kato for their valuable technical assistance. This research was supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (Nos. 17580024 and 23658026).

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Nakajima, R., Otagaki, S., Yamada, K. et al. Molecular cloning and expression analyses of FaFT, FaTFL, and FaAP1 genes in cultivated strawberry: their correlation to flower bud formation. Biol Plant 58, 641–648 (2014). https://doi.org/10.1007/s10535-014-0452-3

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  • DOI: https://doi.org/10.1007/s10535-014-0452-3

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