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Molecular cloning and expression analysis of four actin genes (MiACT) from mango

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

Actin is the most abundant protein in eukaryotic cells and is a key cytoskeletal component controlling cell morphology and motility. In this study, four MiACT genes were isolated from mango by homological cloning and designated as MiACT1, MiACT4, MiACT7, and MiACT9. Sequence alignments and phylogenetic analysis demonstrated that the four MiACT genes of mango were highly similar to each other at the nucleotide and amino acid levels. All of four MiACT proteins showed high similarity to the known actin proteins from other species. Reverse transcription polymerase chain reaction revealed that the four MiACT genes were constitutively and stably expressed in all organs tested. Application of plant growth regulators and four stress treatments had a remarkable effect on the expression of MiACT4, MiACT7 and MiACT9, whereas expression of MiACT1 was unresponsive. In contrast, the expression profiles of the four MiACT genes were not regulated by diurnal rhythms. Moreover, the expression of MiACT1 was not affected by heavy metal treatments and the transcript level of MiACT1 was rather stable in different days during the post-harvest period either under treatment or not. Our results suggest that the four actin genes play important roles throughout the entire life cycle of mango; the constitutively and stably expressed MiACT1 is the best candidate as an internal standard for differential gene expression analysis in mango.

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Abbreviations

ABA:

abscisic acid

BA:

benzyladenine

DAF:

days after full bloom

f-flower:

full flowering period

GA3 :

gibberellic acid

IAA:

indole-3-acetic acid

l-flower:

late flowering period

PP:

paclobutrazol

RT-PCR:

reverse transcription polymerase chain reaction

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Authors and Affiliations

  1. College of Agriculture, Guangxi University, Nanning, 530004, P.R. China

    C. Luo, X. -H. He, H. Chen, Y. Hu & S. -J. Ou

  2. Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Nanning, 530007, P.R. China

    X. -H. He

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  1. C. Luo
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  2. X. -H. He
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  3. H. Chen
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  4. Y. Hu
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  5. S. -J. Ou
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Correspondence to X. -H. He.

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Acknowledgments: This research was supported by Guangxi Natural Science Foundation (2011GXNSFA018115); Program for Excellent Talents in Guangxi Higher Education Institutions (201065); Innovation Project of Guangxi Postgraduate Education (105931001011), and the Scientific Research Foundation of Guangxi University (Grant No. 20090042).

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Luo, C., He, X.H., Chen, H. et al. Molecular cloning and expression analysis of four actin genes (MiACT) from mango. Biol Plant 57, 238–244 (2013). https://doi.org/10.1007/s10535-012-0278-9

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  • DOI: https://doi.org/10.1007/s10535-012-0278-9

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