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The effect of water deficit and excess copper on proline metabolism in Nicotiana benthamiana

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

Fluctuation in proline content is a widespread phenomenon among plants in response to heavy metal stress. To distinguish between the participation of water deficit and copper on changes in proline metabolism, potted plants and floating leaf discs of tobacco were subjected to CuSO4 treatments. The application of copper increased the proline content in the leaves concomitantly with decreased leaf relative water content and increased abscisic acid (ABA) content in the potted plant. Excess copper increased the expression of two proline synthesis genes, pyrroline-5-carboxylate synthetase (P5CS) and ornithine aminotransferase (OAT) and suppressed proline catabolism gene, proline dehydrogenase (PDH). However, in the experiment with tobacco leaf discs floating on CuSO4 solutions, the excess copper decreased proline content and suppressed the expression of the P5CS, OAT and PDH genes. Therefore, proline accumulation in the potted tobacco plants treated with excess Cu treatment might not be the consequence of the increased copper content in tobacco leaves but rather by the accompanied decrease in water content and/or increased ABA content.

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Abbreviations

ABA:

abscisic acid

OAT:

ornithine aminotransferase

P5CS:

pyrroline-5-carboxylate synthetase

PDH:

proline dehydrogenase

RWC:

relative water content

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Acknowledgements

We are grateful to Dr. C.-J. Chang, A. Frary and V. Panwar for critically reviewing and editing the manuscript.

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Authors and Affiliations

  1. Department of Agronomy, National Chung Hsing University, Taichung, 402, Taiwan

    H. -M. Ku & C. -W. Tan

  2. Department of Plant Pathology, National Chung Hsing University, Taichung, 402, Taiwan

    C. -W. Tan & F. -J. Jan

  3. Department of Agriculture Chemistry, National Taiwan University, Taipei, 106, Taiwan

    Y. -S. Su & C. -T. Chen

  4. Biodiversity Research Center, Academia Sinica, Taipei, 115, Taiwan

    C. -Y. Chiu

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  1. H. -M. Ku
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  2. C. -W. Tan
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  5. C. -T. Chen
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Ku, H.M., Tan, C.W., Su, Y.S. et al. The effect of water deficit and excess copper on proline metabolism in Nicotiana benthamiana . Biol Plant 56, 337–343 (2012). https://doi.org/10.1007/s10535-012-0095-1

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  • DOI: https://doi.org/10.1007/s10535-012-0095-1

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