Skip to main content
SpringerLink
Log in

Polyamine content during minimal growth storage of Thymus moroderi explants

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

The polyamine (PA) content of in vitro-grown explants of Thymus moroderi Pau ex Martínez has been evaluated during minimal growth storage. The growth restriction was imposed by the combined action of osmotically-active compounds (15 g dm−3 sucrose and 15 g dm−3 mannitol) present in the Murashige and Skoog medium and the modification of the physical environment of the culture (4 °C and darkness). In these conditions, cultures were maintained up to 29 weeks without subculture. During this storage period, we analyzed contents of free, perchloric acid (PCA)-soluble and PCA-insoluble conjugated PA. Minimal growth storage brought about an increase in free putrescine (Put) coinciding with a reduction in PCA-soluble conjugated Put occurring during the first weeks of storage. PCA-insoluble conjugated spermidine (Spd) also accumulated in response to storage.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

Cad:

cadaverine

Dap:

diaminopropane

FMOC:

N-9-fluorenylmethylchloroformiate

MS:

Murashige and Skoog

PA:

polyamine

PCA:

perchloric acid

Put:

putrescine

Spd:

spermidine

Spm:

spermine

References

  • Alcázar, R., Marco, F., Cuevas, J.C., Patron, M., Ferrando, A., Carrasco, P., Tiburcio, A.F., Altabella, T.: Involvement of polyamines in plant response to abiotic stress. — Biotechnol. Lett. 28: 1867–1876, 2006.

    Article  PubMed  Google Scholar 

  • Aziz, A., Martin-Tanguy, J., Larher, F.: Plasticity of polyamine metabolism associated with high osmotic stress in rape leaf discs and with ethylene treatment. — Plant Growth Regul. 21153–163, 1997.

    Article  CAS  Google Scholar 

  • Carrizo, C.N., Pitta-Alvareza, S.I., Kogan, M.J., Giuliettia, A.M., Tomaro, M.L.: Occurrence of cadaverine in hairy roots of Brugmansia candida. — Phytochemistry 57: 759–763, 2001.

    Article  PubMed  CAS  Google Scholar 

  • Bouchereau, A., Aziz, A., Larher, F., Martin-Tanguy, J.: Polyamines and environmental challenges: recent development. — Plant Sci. 140: 103–125, 1999.

    Article  CAS  Google Scholar 

  • Divakaran, M., Nirmal Babu, K., Peter, K.V.: Conservation of Vanilla species in vitro. — Sci. Hort. 110: 175–180, 2006.

    Article  CAS  Google Scholar 

  • Galzy, R., Compan, D.: Growth and nutrition of grapevine during in vitro long-term storage. — Plant Cell Tissue Organ Cult. 13: 229–237, 1988.

    Article  Google Scholar 

  • Gao, C., Hu, J., Zhang, S., Zheng, Y., Knapp, A.: Association of polyamines in governing the chilling sensitivity of maize genotypes. — Plant Growth Regul. 57: 31–38, 2009.

    Article  CAS  Google Scholar 

  • George, E.F.: Plant propagation by tissue culture. Part 1. The Technology. 2nd Ed. — Exegetics Ltd., Westbury 1993.

    Google Scholar 

  • Gonçalves, S., Romano, A.: In vitro minimum growth for conservation of Drosophyllum lusitanicum. — Biol. Plant. 51: 795–798, 2007.

    Article  Google Scholar 

  • Gopal, J., Chamail, A., Sarkar, D.: Slow-growth in vitro conservation of potato germplasm at normal propagation temperature. — Potato Res. 45: 203–213, 2002.

    Article  Google Scholar 

  • Harding, K.: Molecular stability of the ribosomal RNA genes in Solanum tuberosum plants recovered from slow growth and cryopreservation. — Euphytica 55: 141–146, 1991.

    Article  CAS  Google Scholar 

  • Kaplan, F., Kopka, J., Haskell, D.W., Zhao, W., Schiller, K.C., Gatzke, N., Sung, D.Y., Guy, C.L.: Exploring the temperature-stress metabolome of Arabidopsis. — Plant Physiol. 136: 4159–4168, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Kasukabe, Y., He, L., Nada, K., Misawa, S., Ihara, I., Tachibana, S.: Overexpression of spermidine synthase enhances tolerance to multiple environmental stresses and up-regulates the expression of various stress- regulated genes in transgenic Arabidopsis thaliana. — Plant Cell Physiol. 45: 712–722, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Lee, T.M.: Polyamine regulation of growth and chilling tolerance of rice (Oryza sativa L.) roots cultured in vitro. — Plant Sci. 122: 111–117, 1997.

    Article  CAS  Google Scholar 

  • Liu, J.H., Nakajima, I., Moriguchi, T.: Effects of salt and osmotic stresses on free polyamine content and expression of polyamine biosynthetic genes in Vitis vinifera. — Biol. Plant. 55: 340–344, 2011.

    Article  CAS  Google Scholar 

  • Marco-Medina, A., Casas, J.L., Swennen, R., Panis, B.: Cryopreservation of Thymus moroderi by droplet vitrification. — CryoLetters 31: 14–23, 2010a.

    PubMed  CAS  Google Scholar 

  • Marco-Medina, A., Casas, J.L., González-Benito, M.E.: Comparison of vitrification and encapsulation-dehydration for cryopreservation of Thymus moroderi shoot tips. — CryoLetters 31: 301–309, 2010b.

    PubMed  CAS  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. — Physiol. Plant. 15: 473–497, 1962.

    Article  CAS  Google Scholar 

  • Nair, S., Singh, Z.: Chilling injury in mango fruit in relation to biosynthesis of free polyamines. — J. hort. Sci. Biotechnol. 79: 515–522, 2004.

    CAS  Google Scholar 

  • Negash, A., Krens, F., Schaart, J., Visser, B.: In vitro conservation of enset under slow growth conditions. — Plant Cell Tissue Organ Cult. 66: 107–111, 2001.

    Article  CAS  Google Scholar 

  • Negri, V., Tosti, N., Standardi, A.: Slow-growth storage of single node shoots of apple genotypes. — Plant Cell Tissue Organ Cult. 62: 159–162, 2000.

    Article  Google Scholar 

  • Oufir, M., Legay, S., Nicot, N., Van Moer, K., Hoffmann, L., Renaut, J., Hausman, J.F., Evers, D.: Gene expression in potato during cold exposure: changes in carbohydrate and polyamine metabolisms. — Plant Sci. 175: 839–852, 2008.

    Article  CAS  Google Scholar 

  • Romano, A., Martins-Louçao, M.A.: In vitro cold storage of cork oak shoot cultures. — Plant Cell Tissue Organ Cult. 59: 155–157, 1999.

    Article  Google Scholar 

  • Sarjala, T., Taulavuori, K.: Fluctuation in free and conjugated polyamines in Scots pine seedlings after changes in temperature and daylength. — Acta Physiol. Plant. 26: 271–279, 2004.

    Article  CAS  Google Scholar 

  • Sarkar, D., Chakrabarti, S.K., Naik, P.S.: Slow growth conservation of potato microplants: efficacy of ancymidol for long-term storage in vitro. — Euphytica 117: 133–142, 2001.

    Article  CAS  Google Scholar 

  • Sharma, P., Rajam, M.V.: Spatial and temporal changes in endogenous polyamine levels associated with somatic embryogenesis from different hypocotyl segments of eggplant (Solanum melongena L.). — J. Plant Physiol. 146: 658–664, 1995.

    Article  CAS  Google Scholar 

  • Upreti, K.K., Murti, G.S.R.: Response of grape rootstocks to salinity: changes in root growth, polyamines and abscisic acid. — Biol. Plant. 54: 730–734, 2010.

    Article  CAS  Google Scholar 

  • Yokota, T., Nakayama, M., Harasawa, I., Sato, M., Katsuhara, M., Kawabe, S.: Polyamines, indole-3-acetic acid and abscisic acid in rice phloem sap. — Plant Growth Reg. 15: 125–128, 1994.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plant Biotechnology Laboratory, Institute of Biodiversity CIBIO, University of Alicante, Crta. San Vicente del Raspeig s/n., E-03690, San Vicente del Raspeig, Alicante, Spain

    A. Marco-Medina & J. L. Casas

Authors
  1. A. Marco-Medina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. L. Casas
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. L. Casas.

Additional information

Acknowledgement: A.M.M. was financially supported by a pre-doctoral fellowship from the Spanish Ministry of Science and Innovation.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Marco-Medina, A., Casas, J.L. Polyamine content during minimal growth storage of Thymus moroderi explants. Biol Plant 56, 590–594 (2012). https://doi.org/10.1007/s10535-012-0061-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10535-012-0061-y

Additional key words

Search

Navigation