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Special origin of stem sequence influence the resistance of hairpin expressing plants against PVY

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Biologia Plantarum

Abstract

In this study, 16 hairpin RNA (hpRNA) vectors were constructed, each harboring 50 bp viral RNA sequence as the stem. They all targeted the coat protein (CP) gene of Potato virus Y (PVY). Virus resistance assay revealed that hairpin constructs targeting the anterior 200 bp regions of the CP gene were unable to induce virus resistance, while the 12 hpRNA constructs targeting posterior 600 bp regions induced high virus resistance up to 77.78 %. Northern blot analysis revealed that 50 bp-length hpRNA constructs could be transcribed efficiently and processed into siRNAs; however, no correlation between siRNA accumulation and degree of antiviral defense was observed. Results presented here indicated that the middle and 3′ end of the CP cDNA was important for hpRNA-mediated PVY resistance, improving the design of pathogen-derived hpRNA expression cassettes for transgenic plant against viruses.

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Abbreviations

CaMV:

cauliflower mosaic virus

CB:

carbenicillin

CP:

coat protein

dpi:

day post-inoculation

dsRNA:

double strand RNA

hpRNA:

hairpinRNA

PTGS:

post-transcriptional gene silencing

PVY:

Potato virus Y

SSC:

sodium chloride/sodium citrate

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Acknowledgments

This work was financially supported in part by the National Natural Science Foundation of China (No. 30771408). The first two authors equally participated on the paper.

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, P.R. China

    F. Jiang, B. Wu, C. Zhang, Y. Song, H. An, C. Zhu & F. Wen

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  1. F. Jiang
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  2. B. Wu
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  3. C. Zhang
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  4. Y. Song
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  5. H. An
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  6. C. Zhu
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  7. F. Wen
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Corresponding author

Correspondence to C. Zhu.

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Jiang, F., Wu, B., Zhang, C. et al. Special origin of stem sequence influence the resistance of hairpin expressing plants against PVY. Biol Plant 55, 528–535 (2011). https://doi.org/10.1007/s10535-011-0119-2

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  • DOI: https://doi.org/10.1007/s10535-011-0119-2

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