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Role of auxins, polyamines and ethylene in root formation and growth in sweet orange

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Biologia Plantarum

Abstract

The primary objective of this work was to investigate the role of polyamines (PAs) on root formation and growth in two sweet orange (Citrus sinensis L. Osb.) cultivars Pineapple and Pêra. Adventitious shoots (30-d-old) derived from epicotyl explants were transferred to root induction medium containing Murashige and Skoog salts at different strengths and supplemented with different concentrations and combinations of auxins. Root formation and development decreased in both sweet orange cultivars concomitant with the reduction of medium strength. The α-naphtaleneacetic acid was important during the root differentiation phase, but its combination with indole-3-butyric acid was essential for root elongation. The addition of PAs significantly improved root formation and/or growth, depending on their concentration, whereas the presence of inhibitor of PAs biosynthesis α-difluoromethylornithine (DFMO) inhibited these processes. The rooting impairment caused by DFMO was partially reversed by the supplementation of putrescine. Aminoethoxyvinylglycine AVG and AgNO3 also inhibited in vitro rooting in both sweet orange cultivars, indicating that ethylene was likewise important for rhizogenesis in sweet orange.

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Abbreviations

AVG:

aminoethoxyvinylglycine

BAP:

6-benzylaminopurine

DFMO:

α-difluoromethylornithine

IBA:

indole-3-butyric acid

MS:

Murashige and Skoog

NAA:

α-naphtaleneacetic acid

put:

putrescine

RIM:

rooting induction medium

SIM:

shoot induction medium

Spd:

spermidine

Spm:

spermine

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Acknowledgements

to International Foundation for Science (IFS) for financial support; to The State of Bahia Research Foundation (FAPESB) for financial and fellowship supports; to National Council for Scientific and Technological Development (CNPq) and Coordination of Higher Education and Graduate Training (CAPES) for fellowships support.

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Authors and Affiliations

  1. Center for Biotechnology and Genetics, Biological Sciences Department, State University of Santa Cruz, Ilhéus, 45662-000, Brazil

    A. F. S. Mendes, L. C. Cidade & M. G. C. Costa

  2. Plant Biology Department, Federal University of Viçosa, Viçosa, 36570-000, Brazil

    W. C. Otoni

  3. Embrapa Cassava and Tropical Fruit, Cruz das Almas, 44380-000, Brazil

    W. S. Soares-Filho

Authors
  1. A. F. S. Mendes
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  2. L. C. Cidade
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  3. W. C. Otoni
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  4. W. S. Soares-Filho
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  5. M. G. C. Costa
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Correspondence to M. G. C. Costa.

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Mendes, A.F.S., Cidade, L.C., Otoni, W.C. et al. Role of auxins, polyamines and ethylene in root formation and growth in sweet orange. Biol Plant 55, 375–378 (2011). https://doi.org/10.1007/s10535-011-0058-y

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  • DOI: https://doi.org/10.1007/s10535-011-0058-y

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