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Allelochemical effects on net nitrate uptake and plasma membrane H+-ATPase activity in maize seedlings

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Biologia Plantarum

Abstract

Seven-day-old maize seedlings grown in a nitrogen-free hydroponic culture were exposed for 48 h to 0, 100 and 300 μM trans-cinnamic, p-coumaric, ferulic, caffeic acids, umbelliferone and 200 μM KNO3. Net nitrate uptake was affected by trans-cinnamic, ferulic and p-coumaric acids in a concentration-dependent manner, and trans-cinnamic acid appeared to be the strongest inhibitor. Conversely, at low concentrations, caffeic acid stimulated net nitrate uptake while umbelliferone did not influence it. After 24 h of treatment, plasma membrane H+-ATPase activity significantly decreased in a concentration-dependent manner in response to trans-cinnamic, ferulic and p-coumaric acids, while umbelliferone and caffeic acid had no effect on H+-ATPase activity.

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Abbreviations

BPT:

1,3-bis[tris (hydroxymethyl)-methylamino]-propane

DDT:

1,4-dithioerythritol

EDTA:

ethylenediaminetetraacetic acid

EGTA:

ethyleneglycoltetraacetic acid)

IC50 :

the dose causing 50 % inhibition of the plasma membrane H+-ATPase activity

MES:

2-[N-morpholino]ethanesulphonic acid

NNUR:

net nitrate uptake rate

PMSF:

phenylmethylsulphonyl fluoride

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Authors and Affiliations

  1. Dip. BIOMAA, Facoltà di Agraria, Università Mediterranea di Reggio Calabria, Feo di Vito, I-89124, Reggio Calabria, Italy

    M. R. Abenavoli, A. Lupini, S. Oliva & A. Sorgonà

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  1. M. R. Abenavoli
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  2. A. Lupini
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  3. S. Oliva
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  4. A. Sorgonà
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Correspondence to M. R. Abenavoli.

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Abenavoli, M.R., Lupini, A., Oliva, S. et al. Allelochemical effects on net nitrate uptake and plasma membrane H+-ATPase activity in maize seedlings. Biol Plant 54, 149–153 (2010). https://doi.org/10.1007/s10535-010-0024-0

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  • DOI: https://doi.org/10.1007/s10535-010-0024-0

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