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Population genetic diversity in the polyploid complex of wheatgrasses using isoenzyme and RAPD data

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Biologia Plantarum

Abstract

Thirty five bands (alleles) from six enzyme systems and fifty seven random amplified polymorphic DNA (RAPD) fragments were selected to analyse the genetic diversity of 33 polyploid wheatgrasses (Triticeae) populations of species Thinopyrum junceiforme and Elytrigia pycnantha, and two hybrids, one pentaploid and one novel 9-ploid. Dice’s similarity coefficient, the UPGMA-derived phenograms from RAPD, and allozymes markers showed that the clustering of wheatgrass populations was based on ploidy level. These markers had similar levels of diversity between populations, with high genetic similarity within the same ploidy-level and within population’s individuals. The tetraploid Th. junceiforme populations are closely related, with a large similarity distances varied from 0.8 to 1. Based on the isozyme and RAPD analyses, diploid taxa are related to polyploids with similarity coefficients 0.4.

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Abbreviations

AAT:

aminoaspartate transferase

AMY:

amylase

EST:

esterase

MDH:

malate dehydrogenase

PGI:

phosphoglucose isomerase

PGM:

phosphoglucomutase

RAPD:

random amplified polymorphic DNA

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Author notes

  1. A. Refoufi

    Present address: Faculté des Sciences, Université M’Hamed Bougara, 1 Avenue de l’Indépendance, 35000, Boumerdès, Algerie

Authors and Affiliations

  1. Université de Rennes 1, UMR 6553 Ecobio, Bâtiment 14, Campus de Beaulieu, F-35042, Rennes CEDEX, France

    A. Refoufi & M. A. Esnault

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  1. A. Refoufi
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  2. M. A. Esnault
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Correspondence to A. Refoufi.

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Refoufi, A., Esnault, M.A. Population genetic diversity in the polyploid complex of wheatgrasses using isoenzyme and RAPD data. Biol Plant 52, 543–547 (2008). https://doi.org/10.1007/s10535-008-0106-4

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  • DOI: https://doi.org/10.1007/s10535-008-0106-4

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