Abstract
In this study, bulked segregant analysis (BSA) was used on Larix leptolepis × Larix olgensis hybrids to identify a random amplified polymorphic DNA (RAPD) marker associated with high rooting ability in larch. Two DNA bulks: H (high rooting ability) bulk and L (low rooting ability) bulk were constructed according to the rooting percentages of the stock plants. Among the 328 primers, only S356 could amplify a specific band, named S356445, which only existed in the H bulk and was further confirmed following selective genotyping of individual hybrids. Grounded on the border sequences, S356445 was converted to a sequence characterized amplified region (SCAR) marker, HRL445, which can be useful in marker-assisted selection (MAS) to screen for larch with high rooting ability. All the results strongly indicated that S356445 and HRL445 were closely associated with high rooting ability in larch.
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Abbreviations
- BSA:
-
bulked segregant analysis
- CTAB:
-
cetyltrimethylammonium bromide
- MAS:
-
marker-assisted selection
- PCR:
-
polymerase chain reaction
- RAPD:
-
random amplified polymorphic DNA
- SCAR:
-
sequence characterized amplified region
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Li, H., Zhang, S.G., Gao, J.M. et al. Development of a sequence characterized amplified region (SCAR) marker associated with high rooting ability in Larix . Biol Plant 52, 525–528 (2008). https://doi.org/10.1007/s10535-008-0102-8
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DOI: https://doi.org/10.1007/s10535-008-0102-8