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Water-water cycle involved in dissipation of excess photon energy in phosphorus deficient rice leaves

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Biologia Plantarum

Abstract

The water-water cycle which may be helpful for dissipating the excitation pressure over electron transport chain and minimizing the risk of photoinhibition and photodamage was investigated in rice after 10-d P-deficient treatment. Net photosynthetic rate decreased under P-deficiency, thus the absorption of photon energy exceeded the energy required for CO2 assimilation. A more sensitive response of effective quantum yield of photosystem 2 (ΦPS2) to O2 concentration was observed in plants that suffered P starvation, indicating that more electrons were transported to O2 in the P-deficient leaves. The electron transport rate through photosystem 2 (PS 2) (Jf) was stable, and the fraction of electron transport rate required to sustain CO2 assimilation and photorespiration (Jg/Jf) was significantly decreased accompanied by an increase in the alternative electron transport (Ja/Jf), indicating that a considerable electron amount had been transported to O2 during the water-water cycle in the P-deficient leaves. However, the fraction of electron transport to photorespiration (Jo/Jf) was also increased in the P-deficient leaves and it was less sensitive than that of water-water cycle. Therefore, water-water cycle could serve as an efficient electron sink. The higher non-photochemical fluorescence quenching (qN) in the P-deficient leaves depended on O2 concentration, suggesting that the water-water cycle might also contribute to non-radiative energy dissipation. Hence, the enhanced activity of the water-water cycle is important for protecting photosynthetic apparatus under P-deficiency in rice.

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Abbreviations

APX:

ascorbate peroxidase

ci :

intercellular CO2 concentration

Chl:

chlorophyll

gs :

stomatal conductance

FM:

fresh mass

Ja :

the rate of alternative electron transport

Jf :

the electron transport rate through PS2

Jg :

the rate of electron transport required to maintain photosynthetic carbon reduction cycle (PCR) and photorespiratory carbon oxidation cycle (PCO)

Jo :

the rate of electron transport though photorespiration

MDA:

malonyldialdehyde

O ·−2 :

superoxide radical

PN :

net photosynthetic rate

PCO:

photorespiratory carbon oxidation cycle

PCR:

photosynthetic carbon reduction cycle

PPFD:

photosynthetic photon flux density

Pr:

protein

PS 2:

photosystem 2

qP :

photochemical quenching

qN :

non-photochemical quenching

SOD:

superoxide dismutase

TBA:

barbiturate

TCA:

trichloroacetic acid

ΦPS2 :

effective PS2 quantum yield

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Authors and Affiliations

  1. National Laboratory of Plant Physiology and Biochemistry, Department of Biological Science, College of Life Science, Zhejiang University, Hangzhou, Zhejiang, 310058, China

    X. -Y. Weng, H. -X. Xu, Y. Yang & H. -H. Peng

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  1. X. -Y. Weng
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  2. H. -X. Xu
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  3. Y. Yang
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  4. H. -H. Peng
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Correspondence to X. -Y. Weng.

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Weng, X.Y., Xu, H.X., Yang, Y. et al. Water-water cycle involved in dissipation of excess photon energy in phosphorus deficient rice leaves. Biol Plant 52, 307–313 (2008). https://doi.org/10.1007/s10535-008-0064-x

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  • DOI: https://doi.org/10.1007/s10535-008-0064-x

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