Abstract
Calcium chloride (0.3 or 10 mM) was applied to the growth medium before, together with, or after sodium chloride application, and the effect of the timing of Ca application on the alleviation of salt stress was investigated. Seedlings of maize, tall fescue, and reed canarygrass were grown in medium with 0 and 200 mM NaCl for 5 d. Regardless of the plant species the maximum alleviation of NaCl stress was achieved when CaCl2 and NaCl coexisted in the growth medium. The effects of Ca application were connected with the decrease in the Na content in the plant roots and shoots and increased ATPase activity in the roots.
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References
Aslam, M., Qureshi, R.Q., Ahmed, N.: A rapid screening for salt tolerance in rice (Oryza sativa L.).-Plant Soil 150: 99–107, 1993.
Bradford, M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye-binding.-Anal. Biochem. 72: 248–254, 1976.
Clarkson, D.T., Hanson, J.B.: The mineral nutrition of higher plants.-Annu. Rev. Plant Physiol. 31: 239–298, 1980.
Cramer, G.R., Epstein, E., Lauchli, A.: Na-Ca interactions in barley seedlings: relationship to ion transport and growth.-Plant Cell Environ. 12: 551–558, 1989.
Eklund, L., Eliasson, L.: Effects of calcium ion concentration on cell wall synthesis.-J. exp. Bot. 41: 863–867, 1990.
El-Hamdaoui, A., Redondo-Nieto, M., Torralba, B., Rivilla, R., Bonilla, I., Bolanos, L.: Influence of boron and calcium on the tolerance to salinity of nitrogen-fixing pea plants.-Plant Soil 251: 93–103, 2003.
Gallagher, S.R., Leonard, R.T.: Effect of vanadate, molybdate, and azide on membrane-associated ATPase and soluble phosphatase activities of corn roots.-Plant Physiol. 70: 1335–1340, 1982.
Kinraide, T.B.: Interactions among Ca2+, Na+ and K+ in saline toxicity: quantitative resolution of multiple toxic and ameliorative effects.-J. exp. Bot. 50: 1495–1505, 1999.
Lahaya, P.A., Epstein, E.: Salt toleration by plants: enhancement with calcium.-Science 166: 395–396, 1969.
Lynch, J., Cramer, G.R., Lauchli, A.: Salinity reduces membrane-associated calcium in corn root protoplasts.-Plant Physiol. 83: 390–394, 1987.
MacRobbie, E.A.C.: Functions of ion transport in plant cells and tissues.-Int. Rev. Biochem. 13: 211–247, 1977.
Maeda, Y., Ota, T., Unno, H., Nakazawa, R., Takenaga, H.: Effects of Ca application on reduction of salt stress in seedlings of sheep grass (Aneurolepidium chinense) and reed canarygrass (Phalaris arundinacea L.).-Grassland Sci. 49: 391–394, 2003.
Maeda, Y., Yoshiba, M., Tadano, T.: Comparison of Ca effect on the salt tolerance of suspension cells and intact plants of tobacco (Nicotiana tabacum L., cv. Bright Yellow-2).-Soil Sci. Plant Nutr. 51: 313–318, 2005.
Matoh, T., Kobayashi, M.: Boron and calcium, essential inorganic constituents of pectic polysaccharides in higher plant cell walls.-J. Plant Res. 111: 179–190, 1998.
Murata, Y., Katsura, S., Obi, I., Kakutani, T.: Alterations in Ca2+-binding on plasma membrane after adaptation to salt stress of tobacco cells in suspension.-Plant Cell Physiol. 41: 1286–1292, 2000.
Pitman, M.G.: Ion transport into the xylem.-Annu. Rev. Plant Physiol. 28: 71–88, 1977.
Qui, Q.S., Su, X.F.: The influence of extracellular-side Ca2+ on the activity of the plasma membrane H+-ATPase from wheat roots.-Aust. J. Plant Physiol. 25: 923–928, 1998.
Shachtman, D.P., Munns, R.: Sodium accumulation in leaves of Triticum species that differ in salt tolerance.-Aust. J. Plant Physiol. 19: 331–340, 1992.
Sotiropoulos, T.E.: Effect of NaCl and CaCl2 on growth and contents of minerals, chlorophyll, praline and sugars in the apple rootstock M4 cultured in vitro.-Biol. Plant. 51: 177–180, 2007.
Stassart, J.M., Nerinckz, L., Dejaegere, R.: The interactions between monovalent cations and calcium during their adsorption on isolated cell walls and absorption by intact barley roots.-Ann. Bot. 47: 647–652, 1981.
Yamauchi, T., Hara, T., Sonoda, Y.: Distribution of calcium and boron in the pectin fraction of tomato leaf cell wall.-Plant Cell Physiol. 27: 729–732, 1986.
Yermiyahu, U., Nir, S., Ben-Hayyim, G., Kafkafi, U., Kinraide T.B.: Root elongation in saline solution related to calcium binding to root cell plasma membranes.-Plant Soil 191: 67–76, 1997.
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Maeda, Y., Nakazawa, R. Effects of the timing of calcium application on the alleviation of salt stress in the maize, tall fescue, and reed canarygrass seedlings. Biol Plant 52, 153–156 (2008). https://doi.org/10.1007/s10535-008-0033-4
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DOI: https://doi.org/10.1007/s10535-008-0033-4