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Effects of freezing on plasma membrane H+-ATPase of the callus from Chorispora bungeana

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

The influence of freezing treatment on plasma membrane (PM) H+-ATPase was investigated using plasma membrane vesicles isolated from calluses from Chorispora bungeana Fisch. & C.A. Mey. by the discontinuous sucrose gradient centrifugation. Freezing treatment (−4 °C) for 5 d resulted in significant increases in the ATPase activity and the activity of p-nitrophenyl phosphate (PNPP) hydrolysis, decreases in the Km for ATP hydrolysis and PNPP hydrolysis, and the shift of optimal pH from 6.5 to 7.0. Also, the activity PNPP hydrolysis was less sensitive to vanadate after freezing treatment compared to control, while the inhibition of ATP hydrolysis by hydroxylamine was more sensitive. In addition, freezing treatment also decreased the activation effects of trypsin on PNPP hydrolysis, but increased the activation effects of lysophosphatidylcholine on ATP hydrolysis. Taken together, these results suggested that PM H+-ATPase might play an important role during adaptation to freezing and enhancing the frost hardness in C. bungeana.

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Abbreviations

ATP:

adenosine triphosphate

BSA:

bovine serum albumin

DTT:

dithiothreitol

EDTA:

ethylenediaminetetraacetic acid

lyso-PC:

lysophosphatidylcholine

Mes:

2-(N-morpholino)-ethanesulfonic acid

PM:

plasma membrane

PMSF:

phenylmethylsulfonyl fluoride

PNPP:

p-nitrophenyl phosphate

Tris:

N-tris(hydroxymethyl)-amino methane

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Authors and Affiliations

  1. Key Laboratory of Arid and Pasture Agroecology of Ministry of Education, Lanzhou University, Lanzhou, 730000, Gansu, P.R. China

    J. M. Wu, Z. G. Zhao, H. P. Guo, W. X. Li, L. Z. An & S. J. Xu

  2. Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Science, Lanzhou, 730000, Gansu, P.R. China

    L. Z. An & T. Chen

  3. College of Agronomy, Gansu Agriculture University, Lanzhou, 730070, Gansu, P.R. China

    H. Xing

Authors
  1. J. M. Wu
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  2. Z. G. Zhao
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  3. H. Xing
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  4. H. P. Guo
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  5. W. X. Li
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  6. L. Z. An
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  7. S. J. Xu
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  8. T. Chen
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Correspondence to L. Z. An.

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Wu, J.M., Zhao, Z.G., Xing, H. et al. Effects of freezing on plasma membrane H+-ATPase of the callus from Chorispora bungeana . Biol Plant 51, 229–234 (2007). https://doi.org/10.1007/s10535-007-0046-4

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  • DOI: https://doi.org/10.1007/s10535-007-0046-4

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