Abstract
During the entire period of internode growth of Merremia emarginata contents of gibberellic acid (GA3), phenyl-acetic acid (PAA), indole-3-acetic acid (IAA, free and conjugated) and abscisic acid (ABA, free and conjugated) were estimated by ELISA using polyclonal antibodies raised against each hormones. At the time of internode elongation free auxin content was low and increased with the decrease in the rate of elongation. In contrast, conjugated IAA showed declining trend where free IAA content was remarkably high, suggesting thereby that conjugated IAA might have mobilized during the later phase of internode development. The endogenous GA3 contents were high as compared to other hormones; however, no significant role of GA3 was discernible in elongation growth. Conjugated ABA contents remained very low during the elongation growth and increased thereafter.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- ABA:
-
abscisic acid
- ELISA:
-
enzyme link immunonosorbant assay
- GA3:
-
gibberellic acid
- IAA:
-
indole-3-acetic acid
- PAA:
-
phenyl-acetic acid
References
Anker, L.: Auxin synthesis inhibition by abscisic acid and its reversal by gibberellic acid.-Acta bot. neerl. 24: 339–347, 1975.
Bandurski, R.S., Schulze, A.: Concentration of indole-3-acetic acid and its esters in plants.-Plant Physiol. 60: 211–213, 1977.
Cohen, J.D., Bandurski, R.S.: Chemistry and physiology of the bound auxin.-Annu. Rev. Plant Physiol. 33: 403–430, 1982.
Cosgrove, D.J.: How do plant cell walls expand?-Plant Physiol. 102: 1–6, 1993.
Fujioka, S., Yamane, H., Spray, C.R., Phinney, B.O., Gaskin, P., MacMillan, J., Takahashi, N.: Gibberellin A3 is biosynthesized from gibberellin A20 via gibberellin A5 in shoots of Zea mays L.-Plant Physiol. 94: 127–131, 1990.
Gokani, S.J., Kumar, R., Thaker, V.S.: Potential role of abscisic acid in cotton fiber and ovule development.-J. Plant Growth Regul. 17: 1–5, 1998.
Gokani, S.J., Thaker, V.S.: Accumulation of abscisic acid in cotton fiber and seed of normal and abnormal bolls.-J. agr. Sci. 137: 445–451, 2001.
Gokani, S.J., Thaker, V.S.: Physiological and biochemical changes associated with cotton fiber development. IX. Role of IAA and PAA.-Field Crops Res. 77: 127–136, 2002.
Kara, A.N., Kotov, A.A., Bukhov, N.G.: Specific distribution of gibberellins, cytokinins, indole-3-acetic acid and abscisic acid in radish plants closely correlates with photomorphogenetic responses to blue or red light.-J. Plant Physiol. 151: 51–59, 1997.
Kaufman, P.B., Jones, R.A.: Regulation of growth in Avena (oat) stem segments by gibberellic acid and abscisic acid.-Physiol. Plant. 31: 39–43, 1974.
Lüthen, H., Bigdon, M., Böttger, M.: Reexamination of acid growth theory of auxin action.-Plant Physiol. 93: 931–939, 1990.
Millborrow, B.V.: Interaction of plant hormones. Abscisic acid and gibberellic acid.-Science 168: 875–877, 1970.
Miyamoto, K., Kamisaka, S.: Stimulation of Pisum sativum epicotyl elongation by gibberellin and auxin. Different effects of two hormones on osmoregulation and cell walls.-Physiol. Plant. 74: 457–466, 1988.
Pilet, P.E.: Abscisic acid as a root growth inhibitor: physiological analysis.-Planta 122: 299–302, 1975a.
Pilet, P.E.: Action of fusicoccin and abscisic acid on root growth.-Plant Sci. Lett. 5: 137–140, 1975b.
Rabadia, V.S., Thaker, V.S., Singh, Y.D.: Relationship between water content and growth of seed and fiber in three cotton genotypes.-J. Agr. Crop Sci. 183: 255–261, 1999.
Rayle, D.L., Cleland, R.E.: The acid growth theory of auxin-induced cell elongation is alive and well.-Plant Physiol. 99: 1271–1274, 1992.
Sharma, R.K., Thaker, V.S.: Quantification of abscisic acid in cotton leaf by indirect enzyme immunoassay.-J. Sci. agr. Res. 62: 21–26, 2001.
Turusaki, K., Masuda, Y., Sakurai, N.: Distribution of indole-3-acetic acid in apoplast and symplast of squash (Cucurbita maxima) hypocotyls.-Plant Cell Physiol. 38: 352–356, 1997.
Wightman, F., Lighty, D.L.: Identification of phenyl acetic acid as a natural auxin in the shoots of higher plants.-Physiol. Plant. 55: 17–24, 1982.
Wightman, F., Schneider, E.A., Thimann, K.V.: Hormonal factors controlling the initiation and development of lateral roots. II. Effects of exogenous growth factors on lateral root formation in pea roots.-Physiol. Plant. 49: 304–314, 1980.
Yang, T., Davies, P.J., Reid, J.B.: Genetic dissection of the relative roles of auxin and gibberellin in the regulation of stem elongation in intact light-grown peas.-Plant Physiol. 110: 1029–1034, 1996.
Yang, T., Law, D.M., Davies, P.J.: Magnitude and kinetics of stem elongation induced by exogenous indole-3-acetic acid in intact light grown pea seedlings.-Plant Physiol. 102: 717–724, 1993.
Yaxley, J.R., Ross, J.J., Sherrieff, L.J., Reid, J.B.: Gibberellin biosynthesis mutations and root development in pea.-Plant Physiol. 125: 935–942, 2001.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Patel, D., Thaker, V.S. Estimation of endogenous contents of phytohormones during internode development in Merremia emarginata . Biol Plant 51, 75–79 (2007). https://doi.org/10.1007/s10535-007-0015-y
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10535-007-0015-y