Abstract
The effect of sucrose on fruiting, seed production, and seed germination of lesser centaury [Centaurium pulchellum (Sw.) Druce] was examined using explants of flowers and flower buds. Sucrose concentrations in the culture medium ranged from 0.003 to 0.3 M. It has been shown that the number of auxiliary buds, capsules dimension, number of viable seeds per capsule and seed dimensions increased with the increase of sucrose concentrations. The highest values were recorded at sucrose concentrations higher than 0.03 M, except for seeds size, which were larger at sucrose concentration ranging from 0.003 to 0.1 M. The germination of in vitro produced seeds was affected by previous culture history: a higher germination percentage was obtained in seeds that were raised from explants originally grown on medium with sucrose concentrations higher than 0.003 M.
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Abbreviations
- F:
-
flowers
- FB:
-
flower buds
- GA3 :
-
gibberellic acid
References
Al-Khayri, J.M., Huang, F.H., Morelock, T.E.: In vitro flowering in regenerated shoots of spinach.-HortScience 26: 1422, 1991.
Bernier, G., Lejeune, P., Kinet, J. M.: Sucrose levels in leaf exudate as a function of floral induction in the long day plant Sinapis alba.-Plant Physiol. Biochem. 29: 153–157, 1991.
Cheng, W.H., Chourey, P.S.: Genetic evidence that invertase-mediated release of hexoses is critical for appropriate carbon partitioning and normal seed development in maize.-Theor. appl. Genet. 98: 485–495, 1999.
D’Aoust, M.A., Yelle, S., Nguyen-Quoc, B.: Antisense inhibition of tomato fruit sucrose synthase decreases fruit setting and the sucrose unloading capacity of young fruit.-Plant Cell 11: 2407–2418, 1999.
Focks, N., Benning, C.: Wrincled1: a novel, low-seed-oil mutant of Arabidopsis with a deficiency in seed-specific regulation of carbohydrate metabolism.-Plant Physiol. 118: 91–101, 1998.
Gazzarrini, S., McCourt, P.: Genetic interactions between ABA, ethylene and sugar signaling pathways.-Curr. Opin. Plant Biol. 4: 387–391, 2001.
Janković, T., Krstić, D., Šavikin-Fodulović, K., Menković, N., Grubišić, D.: Xanthones and secoiridoids from hairy root cultures of Centaurium erythraea and Centaurium pulchellum.-Planta med. 68: 944–946, 2002.
Ket, N.V., Hahn, E.J., Park, S.Y., Chakrabarty, D., Peak, K.Y.: Micropropagation of an endangered orchid Anoectochilus formosanus.-Biol. Plant. 48: 339–344, 2004.
Krstić, D., Janković, T., Šavikin-Fodulović, K., Menković, N., Grubišić, D.: Secoiridoids and xathones in the shoots and roots of Centaurium pulchellum cultured in vitro.-In Vitro cell. dev. Biol. Plant. 39: 203–207, 2003.
Loreti, E., De Bellis, L., Alpi, A., Perata, P.: Why and how do plant cells sense sugars?-Ann. Bot. 88: 803–812, 2001.
Lunn, L.E., MacRae, E.: New complexities in the synthesis of sucrose.-Curr. Opin. Plant Biol. 6: 208–214, 2003.
Micallef, B.J., Haskins, K.A., Vanderveer, P.J., Roh, K.S., Shewmaker, C.K., Sharkey, T.D.: Altered photosynthesis, flowering, and fruiting in transgenic tomato plants that have an increased capacity for sucrose synthesis.-Planta 196: 327–334, 1995.
Miller, M.E., Chourey, P.S.: The maize invertaze-deficient miniature-1 seed mutation is associated with aberrant pedicel and endosperm development.-Plant Cell 4: 297–305, 1992.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473–497, 1962.
Ohto, M.A., Onai, K., Furukawa, Y., Aoki, E., Araki, T., Nakamura, K.: Effects of sugar on vegetative development and floral transition in Arabidopsis.-Plant Physiol. 127: 252–261, 2001.
Perata, P., Matsukura, C., Vernieri, P., Yamaguchi, J.: Sugar repression of a gibberellin-dependent signaling pathway in barley embryos.-Plant Cell 9: 2197–2208, 1997.
Perilleux, C., Bernier, G.: Leaf carbohydrate status in Lolium temulentum during the induction of flowering.-New Phytol. 135: 59–66, 1997.
Roldan, M., Gomez-Mena, C., Ruiz-Garcia, L., Salinas, J., Martinez-Zapater, J.M.: Sucrose availability on the aerial part of the plant promoters morphogenesis and flowering of Arabidopsis in the dark.-Plant J. 20: 581–590, 1999.
Shiau, Y.J., Nalawade, S.M., Hsai, C.N., Tsay, H.S.: Propagation of Haemaria discolor via in vitro seed germination.-Biol. Plant. 49: 341–346, 2005.
Tisserat, B., Galletta, P.D.: In vitro flowering of Capsicum fruitescens L.-HortScience 30: 130–132, 1995.
Weber, H., Borisjuk, L., Wobus, U.: Sugar import and metabolism during seed development.-Trends Plant Sci. 2: 169–174, 1997.
Weber, H., Heim, U., Golombek, S., Borisjuk, Lj., Wobus, U.: Assimilate uptake and the regulation of seed development.-Seed Sci. Res. 8: 331–345, 1998.
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Todorović, S., Grubišić, D., Giba, Z. et al. Sucrose effects on in vitro fruiting and seed production of Centaurium pulchellum . Biol Plant 50, 771–774 (2006). https://doi.org/10.1007/s10535-006-0128-8
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DOI: https://doi.org/10.1007/s10535-006-0128-8