Abstract
Plants of Miscanthus sinensis (cv. Giganteus) were grown in hydroponics for three months in nutrient solution with 0, 2.2, 4.4 and 6.6 μM CdNO3. Growth parameters, catalase (CAT), guaiacol peroxidase (POD), ascorbate peroxidase (APX) and superoxide dismutase (SOD) activities were analysed in leaves and roots collected after 1-and 3-month exposure. Dry biomass of all miscanthus organs was affected by Cd concentration both after 1-and 3-month exposure. No visible symptoms of Cd toxicity were observed in shoots and rhizomes of plants grown in presence of Cd. In contrast, roots became shorter and thicker and the whole root system more dense and compact already after one month of treatment with 6.6 μM Cd. The lower Cd concentration increased the enzymes activities after 3 months in leaves and only after 1-month in roots, while a decrease in activity was observed at higher Cd concentrations.
This is a preview of subscription content, log in via an institution to check access.
Abbreviations
- APX:
-
ascorbate peroxidase
- CAT:
-
catalase
- POD:
-
guaiacol peroxidase
- SOD:
-
superoxide dismutase
References
Aebi, H.: Catalase in vitro.-Methods Enzymol. 105: 121–126, 1984.
Arduini, I., Masoni, A., Ercoli, L., Mariotti, M.: Growth and cadmium uptake of Miscanthus sinensis as affected by cadmium.-Agr. Med. 133: 169–178, 2003.
Arduini, I., Masoni, A., Mariotti, M., Ercoli, L.: Low cadmium application increase miscanthus growth and cadmium translocation-Environ. exp. Bot. 52: 153–164, 2004.
Astolfi, S., Zuchi, S., Passera, C.: Effects of cadmium on the metabolic activity of Avena sativa plants grown in soil or hydroponics culture.-Biol. Plant. 48: 413–418, 2004.
Baszynski, T., Wajda, L., Krol, M., Wolinska, D., Krupa, Z., Tukendorf, A.: Photosynthetic activities of cadmium-treated tomato plants.-Physiol. Plant. 63: 293–298, 1980.
Chance, B.,A., Maehly, C.: Assay of catalase and peroxidase.-In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Pp. 764–775. Academic Press, New York 1955.
Chaoui, A., Mazhoudi, S., Ghorbal, M.H., El Ferjani, E.: Cadmium and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in bean (Phaseolus vulgaris L.).-Plant Sci. 127:139–147, 1997.
Chen, G.X., Asada, K.: Ascorbate peroxidase in tea leaves: occurrence of two isoforms and the differences in their enzymatic properties.-Plant Cell Physiol. 30: 987–998, 1989.
Chen, Y.X., He, Y.F., Luo, Y.M., Yu, Y.L., Lin, Q., Wong, M.H.: Physiological mechanism of plant roots exposed to cadmium.-Chemosphere 50: 789–793, 2003.
Clark, R.B.: Nutrient solution growth of sorghum and corn in mineral nutrition studies.-J. Plant Nutr. 5: 1039–1057, 1982.
Ercoli, L., Mariotti, M., Masoni, A., Bonari, E.: Effect of irrigation and nitrogen fertilization on biomass yield and efficiency of energy use in crop production of Miscanthus.-Field Crops Res. 63: 3–11, 1999.
Gallego, S.M., Benavides, M.P., Tomaro, M.L.: Effect of heavy metal ion excess on sunflower leaves: evidence for involvement of oxidative stress.-Plant Sci. 121: 151–159, 1996.
Hardyman, R.T., Jacoby, B.: Absorption and translocation of Cd in bush beans (Phaseolus vulgaris).-Physiol. Plant. 61: 670–674, 1984.
Himken, M., Lammel, J., Neukirchen, D., Czyionka-Krause, U., Olfs, H.W.: Cultivation of Miscanthus under west European conditions: seasonal changes in dry matter production, nutrient uptake and remobilization.-Plant Soil 189: 117–126, 1997.
Kevrešan, S., Kiršek, S., Kandraç, J., Petrović, N., Kelemen, Dj.; Dynamics of cadmium distribution in the intercellular space and inside cells in soybean roots, stem and leaves-Biol. Plant. 46: 85–88, 2003.
Lozano-Rodriguez, E., Hernandez, L.E., Bonay, P., Carpena-Ruiz, R.O.: Distribution of cadmium in shoot and root tissues of maize and pea plants: physiological disturbances.-J. exp. Bot. 48: 123–128, 1997.
Madamanchi, N.R., Donahue, J.L., Cramer, C.L., Alscher, R.G., Pedersen, K.: Differential response of Cu, Zn superoxide dismutases in two pea cultivars during a short-term exposure to sulfur dioxide.-Plant mol. Biol. 26: 95–103, 1994.
Rivetta, A., Negrini, N., Cocucci, M.: Involvement of Ca2+-calmodulin in Cd2+ toxicity during the early phases of radish (Raphanus sativus L.) seed germination.-Plant Cell Environ. 20: 600–608, 1997.
Sandalio, L.M., Dalurzo, H.C., Gómez, M., Romero-Puertas, M.C., Del Rio, L.A.: Cadmium-induced changes in the growth and oxidative metabolism of pea plants.-J. exp. Bot. 52: 2115–2126, 2001.
Sanità di Toppi, L., Gabbrielli, R.: Response to cadmium in higher plants.-Environ. exp. Bot. 41: 105–130, 1999.
Schützendübel, A., Polle, A.: Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization.-J. exp. Bot. 53: 1351–1365, 2002.
Schwarz, H.: Miscanthus sinensis Giganteus production on several sites in Austria.-Biomass Bioenergy 5: 413–419, 1993.
Shaw, B.P.: Effects of mercury and cadmium on the activities of antioxidative enzymes in the seedlings of Phaseolus aureus.-Biol. Plant. 37: 587–596, 1995.
Skórzyńska-Polit E., Drążkiewicz, M., Krupa Z.: The activity of the antioxidative system in cadmium-treated Arabidopsis thaliana.-Biol. Plant. 47: 71–78, 2003/4.
Šottníková, A., Lunáčková, L., Masarovičová, E., Lux, A., Streško, V.: Changes in the rooting and growth of willows and poplars induced by cadmiun.-Biol. Plant. 46: 129–131, 2003.
Streb, P., Michael-Knauf, A., Feierabend, J.: Preferential photoinactivation of catalase and photoinhibition of photosystem II are common early symptoms under various osmotic and chemical stress conditions.-Physiol. Plant. 88: 590–598, 1993.
Van Assche, F., Clijsters, H.: Effects of metals on enzyme activity in plants.-Plant Cell Environ. 13: 195–206, 1990.
Wagner, G.J.: Accumulation of cadmium in crop plants and its consequences to human health.-Adv. Agron. 51: 173–212, 1994.
Wu, F., Zhang, G., Dominy, P.: Four barley genotypes respond differently to cadmium: lipid peroxidation and activities of antioxidant capacity.-Environ. Exp. Bot. 50: 67–78, 2003.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Scebba, F., Arduini, I., Ercoli, L. et al. Cadmium effects on growth and antioxidant enzymes activities in Miscanthus sinensis . Biol Plant 50, 688–692 (2006). https://doi.org/10.1007/s10535-006-0107-0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10535-006-0107-0