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Pyoverdine and histicorrugatin-mediated iron acquisition in Pseudomonas thivervalensis

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Abstract

The genome of Pseudomonas thivervalensis LMG 21626T has been sequenced and a genomic, genetic and structural analysis of the siderophore mediated iron acquisition was undertaken. Pseudomonas thivervalensis produces two structurally new siderophores, pyoverdine PYOthi which is typical for P. thivervalensis strains and a closely related strain, and the lipopeptidic siderophore histicorrugatin which is also detected in P. lini. Histicorrugatin consists out of an eight amino acid long peptide which is linked to octanoic acid. It is structurally related to the siderophores corrugatin and ornicorrugatin. Analysis of the proteome for TonB-dependent receptors identified 25 candidates. Comparison of the TonB-dependent receptors of P. thivervalensis with the 17 receptors of its phylogenetic neighbor, P. brassicacearum subsp. brassicacearum NFM 421, showed that NFM 421 shares the same set of receptors with LMG 21626T, including the histicorrugatin receptor. An exception was found for their cognate pyoverdine receptor which can be explained by the observation that both strains produce structurally different pyoverdines. Mass analysis showed that NFM 421 did not produce histicorrugatin, but the analogue ornicorrugatin. Growth stimulation assays with a variety of structurally distinct pyoverdines produced by other Pseudomonas species demonstrated that LMG 21626T and NFM 421 are able to utilize almost the same set of pyoverdines. Strain NFM 421 is able utilize two additional pyoverdines, pyoverdine of P. fluorescens Pf0–1 and P. citronellolis LMG 18378T, these pyoverdines are probably taken up by the FpvA receptor of NFM 421.

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Acknowledgments

The authors are indebted to Pelletier Dale, Baldwin Ian-Thomas and Gasser Ilona for generous gift of strains.

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Authors and Affiliations

  1. Institut de Recherches Microbiologiques-Wiame, Campus du CERIA, 1 avenue Emile Gryson, bât 4B, 1070, Brussels, Belgium

    Sandra Matthijs & Nathalie Brandt

  2. Walloon Center for Industrial Biology, University of Liège/Gembloux Agro-Bio Tech, Gembloux, Belgium

    Marc Ongena

  3. Laboratory of Microbial Ecology of the Rhizosphere and Extreme Environments (LEMIRE), Aix-Marseille Université, CEA, CNRS, UMR 7265 Biosciences and biotechnology Institute of Aix-Marseille (BIAM), ECCOREV FR 3098, CEA/Cadarache, St-Paul-Lez-Durance, France

    Wafa Achouak

  4. Département Génétique Moléculaire, Génomique, Microbiologie, UMR, 7156, CNRS/Université Louis-Pasteur, 28 rue Goethe, 67000, Strasbourg, France

    Jean-Marie Meyer

  5. Institut für Organische Chemie, Universität zu Köln, Greinstrasse 4, 50939, Cologne, Germany

    Herbert Budzikiewicz

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  1. Sandra Matthijs
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  2. Nathalie Brandt
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  4. Wafa Achouak
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Correspondence to Sandra Matthijs.

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Matthijs, S., Brandt, N., Ongena, M. et al. Pyoverdine and histicorrugatin-mediated iron acquisition in Pseudomonas thivervalensis . Biometals 29, 467–485 (2016). https://doi.org/10.1007/s10534-016-9929-1

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