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Investigation of metal sorption behavior of Slp1 from Lysinibacillus sphaericus JG-B53: a combined study using QCM-D, ICP-MS and AFM

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Abstract

Surface layer proteins (S-layer) of Lysinibacillus sphaericus JG-B53 are biological compounds with several bio-based technical applications such as biosorptive materials for metal removal or rare metals recovery from the environment. Despite their well-described applications, a deeper understanding of their metal sorption behavior still remains challenging. The metal sorption ability of Au3+, Pd2+, Pt2+ and Eu3+ was investigated by ICP-MS, AFM and QCM-D which enables the sorption detection in real-time during in situ experiments. Results indicate a high binding of Pd, followed by Au, Eu and Pt to the proteins. The comparison between different methods allowed a deeper understanding of the metal sorption of isolated S-layer either frees in liquid, adsorbed forming a protein layer or as the bacteria surface.

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Acknowledgments

The present work was partially funded by the BMBF-project BIONA (BMBF/DLR 01RB0805A). The author of this paper would like to thank Aline Ritter for her valuable support with ICP-MS measurements. Special thanks to Prof. E. Brunner (TU Dresden), Prof. J.-U. Ackermann (HTW Dresden).

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Authors and Affiliations

  1. Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf, Bautzner Landstrasse 400, 01328, Dresden, Germany

    Matthias Suhr, Nancy Unger, Karen E. Viacava & Johannes Raff

  2. Helmholtz-Institute Freiberg for Resource Technology, Helmholtz-Zentrum Dresden-Rossendorf, Bautzner Landstrasse 400, 01328, Dresden, Germany

    Tobias J. Günther, Johannes Raff & Katrin Pollmann

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  1. Matthias Suhr
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  2. Nancy Unger
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  3. Karen E. Viacava
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  4. Tobias J. Günther
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  5. Johannes Raff
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  6. Katrin Pollmann
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Correspondence to Matthias Suhr.

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Suhr, M., Unger, N., Viacava, K.E. et al. Investigation of metal sorption behavior of Slp1 from Lysinibacillus sphaericus JG-B53: a combined study using QCM-D, ICP-MS and AFM. Biometals 27, 1337–1349 (2014). https://doi.org/10.1007/s10534-014-9794-8

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