Skip to main content
SpringerLink
Log in

Protective role of l-ascorbic acid on antioxidant defense system in erythrocytes of albino rats exposed to nickel sulfate

  • OriginalPaper
  • Published:
BioMetals Aims and scope Submit manuscript

Abstract

In this experimental study, we investigated whether l-ascorbic acid has any influence on the blood antioxidant defense system, lipid peroxidation and hematological parameters of the albino rats exposed to nickel sulfate(NiSO4).Twenty four adult rats were divided into four groups of six animals in each group. The control rats were untreated and comprised Group I. Group II rats were administered nickel sulfate (2.0 mg/100 g b.wt.; intraperitonially, i.p.). Group II rats were treated orally l-ascorbic acid (50 mg/100 g b.wt.) and Group IV rats were given both nickel sulfate and l-ascorbic acid simultaneously on alternate days until the tenth dose. The hematological parameters were assessed: red blood corpuscle counts, packed cell volume %, hemoglobin concentration, white blood corpuscle counts and platelets count decreased significantly and clotting time increased significantly in nickel treated rats. We also observed increase malondialdehyde (MDA) and decrease glutathione level (GSH) in erythrocytes of nickel treated rats. The activities of erythrocyte antioxidant enzymes like superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) were significantly increased in rats treated with nickel sulfate. Simultaneously treatment of l-ascorbic acid exhibited a possible protective role on the toxic effect of nickel sulfate on the hematological values, erythrocyte MDA and GSH concentrations as well as antioxidant enzymatic defense system.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aebi H (1974) Catalase. In: Bergmeyer HU (ed) Methods of Enzymatic Analyses. Weinheim, Verlag Chemie, pp 673 – 684

    Google Scholar 

  • Agency for Toxic Substances and Disease Registry (ATSDR). 2003 Toxicological profile for nickel, US department of Health and Human Services, Public Health Service, ATSDR. Atlanta, Georgia, USA: US Government Printing

  • Ambrose AM, Larson PS, Boezelleca JF et al 1976 Long term toxicologic assessment of nickel in rats and dogs. J Food Sci Technol 13, 181–187

    Google Scholar 

  • Beutler E, Duron O, Kelly BM (1963) Improved method for the determination of blood glutathione. J Lab Clin Med 61:882–888

    PubMed  CAS  Google Scholar 

  • Bordes E, Papillion VV (1983) Myocardial change induced by nickel and in association with cadmium. Rev Ig Bacteriol Virusal Parazitol Epidemol Pneumotizol 32:51–56

    Google Scholar 

  • Brucka-Jastrz E, Bska M, Protas-Owicki A (2005) Effects of cadmium and nickel exposure on hematological parameters of common carp, Cyprinus carpio L. ACTA Ichthyologica et piscatora 35(1):29–38

    Google Scholar 

  • Costa M, Klein CB (1999) Nickel carcinogenesis mutation, epigenetics,or selection. Environ Health Perspect 107(9):A438-A439

    Article  PubMed  CAS  Google Scholar 

  • Das KK, Dasgupta S (1997) Alteration of testicular biochemistry during protein restriction in nickel treated rats. Biol Trace Elem Res 60:243–248

    PubMed  CAS  Google Scholar 

  • Das KK, Dasgupta S (1998) Studies on the role of nickel in the metabolism of ascorbic acid and cholesterol in experimental animals. Ind J Physiol Allied Sci 52:58–62

    CAS  Google Scholar 

  • Das KK, Dasgupta S (2000) Effect of nickel on testicular nucleic acid concentrations of rats on protein restriction. Biol Trace Elem Res 73:175–180

    Article  PubMed  CAS  Google Scholar 

  • Das KK, Das SN, Dasgupta S (2001) The influence of ascorbic acid on nickel induced hepatic lipid peroxidation in rats. J Basic Clin Physiol Pharmacol 12:187- 194

    PubMed  CAS  Google Scholar 

  • Das KK, Dasgupta S (2002) Effect of nickel sulfate on testicular steroidogenesis in rats during protein restriction. Environ Health Perspect 110: 923–926

    PubMed  CAS  Google Scholar 

  • Das KK, Das SN (2004) Studies on the role of ascorbic acid on nickel induced hepatic nucleic acid concentrations in rats. J Basic Clin Physiol Pharmacol 14(3–4):185–195

    Google Scholar 

  • Das KK, Das Gupta A, Dhundasi SA, Patil AM, Das SN, Ambekar JG (2006) Effect of l-ascorbic acid on nickel-induced alteration in serum lipid profiles and liver histopathology in rats. J Basic Clin Physiol Pharmacol 17(1):29–44

    PubMed  CAS  Google Scholar 

  • Declaration of Helsinki (1964) 1996 Amended by World Medical Assembly, Venice, Italy, 1983. Br Med J 313(70):1448–1449

    Google Scholar 

  • Doreswamy K, Balakrishna S, Thimappa R, Muralidhara (2004) Nickel-induced oxidative stress in testis of mice: evidence of DNA damage and genotoxic effects. J Androl 25:996–1003

    PubMed  CAS  Google Scholar 

  • Dunnick JK, Elwell MR, Radovsky AE et al (1995) Comparative carcinogenic effects of nickel subsulfide, nickel oxide, or nickel sulfate chronic exposure in the lung. Cancer Res 55:5251–5256

    PubMed  CAS  Google Scholar 

  • Edes I, Piros G, Toth GK, Csanady M, Kovacs K (1986) Effect of long term alcohol ingestion on glutathione metabolizing enzymes and taurine content in the myocardium of rats. Cardiovasc.Res 20:918–921

    Article  PubMed  CAS  Google Scholar 

  • Ercal N, Gurer O, Aykin-Burns N (2001) Toxic metals and oxidative stress part 1: mechanisms involved in metal induced oxidative damage. Curr Top Med Chem 1:529–539

    Article  PubMed  CAS  Google Scholar 

  • Garcia-Mazano A, Gonzalez-Llaven J, Lemini C et al (2001) Standardization of rat blood clotting tests with reagent used for humans. Proc West Pharmacol Soc 44:153 – 155

    Google Scholar 

  • Gowenlock AH (1996) Varley’s practical clinical biochemistry, 6th edition (Indian reprint). New Delhi, CBS Publishers

    Google Scholar 

  • Gutteridge JMC(1993) Free radicals in disease processes: a complication of cause and consequence. Free Radic Res Commun 19:141–158

    PubMed  CAS  Google Scholar 

  • Halliwell B, Gutteridge JMC (1984) Lipid peroxidation, oxygen radicals, cell damage, and antioxidant therapy. Lancet 1:1396-1397

    Article  PubMed  CAS  Google Scholar 

  • Halliwell B, Gutteridge JMC (1986) Oxygen free radicals and iron in relation to biology and medicine: some problem and concepts. Arch Biochem Biophys 246:501-514

    Article  PubMed  CAS  Google Scholar 

  • Hussain KH, Sugenetran K, Pant SC et al (1992) Biochemical and pathological change in response to hypoxic and protection by antioxidant in rats. Indian J Physiol Pharmacol 36:51–56

    Google Scholar 

  • Kalinih JF, Emond CA, Dalton TK et al (2005) Embedded weapons grade tungsten alloy shrapnel rapidly induces metastasis high grade rhabdomyo-sarcomas in F344 rats. Env Health Perspect 113(6):729 – 734

    Article  CAS  Google Scholar 

  • Kanungo MS, Patnaik BK (1964) Ascorbic acid and ageing in the ratio: uptake of ascorbic acid by skin and bone marrow, and its concentration in various organs. Biochem J 90:637

    PubMed  CAS  Google Scholar 

  • Kelle M, Diken H, Sermet AR, Atmaca M, Tomer C (1999) Effect of exercise on blood antioxidant status and erythrocute lipid peroxidation: Role of dietery supplementation of vitamin E. Tr J Med Sci 29:95–100

    CAS  Google Scholar 

  • Kei S (1978) Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin Chem Acta 90:37–43

    Article  Google Scholar 

  • Kupferschmidt H. 2001 (May 16–19) Features and management of nickel intoxication. In: XXI international congress of the EAPCCT. Barcelona

  • Kurata M, Suzuki M, Agar NS (1993) Antioxidant systems and erythrocytes life-span in mammals. Comp Biochem Physiol B 106:477–487

    Article  PubMed  CAS  Google Scholar 

  • Mates JM (2000) Effect of antioxidants enzymes in the molecular control of reactive oxygen species. Toxicology 153:83–104

    Article  PubMed  CAS  Google Scholar 

  • Merchant MA, Mod DN (2004) Acute and chronic effects of aspirin on hematological parameters and hepatic ferritin expression in mice. Indian J Pharmacol 36(4):226–230

    CAS  Google Scholar 

  • Misra HP, Fridovich I (1972) The role of superoxide anion in the autooxidation of epinephrine and simple assay for superoxide dismutase. J Bio Chem 247:3170–3175

    CAS  Google Scholar 

  • Nakababu E, Chrest FJ, Rifkind JM (2003) Hydrogen peroxide induced heme degradation in red blood cell: the protective roles of catalase and glutathione peroxidase. Biochem Biophys Acta 1620:211–217

    Google Scholar 

  • Nielsen FH, Shuler TR, Mcleod TG, Zimmerman TJ (1984) Nickel influences iron metabolism through physiologic, pharmacologic and toxicologic mechanisms in rats. J Nutr 114:1280–1288

    PubMed  CAS  Google Scholar 

  • Nieboer E, Nriagu JO. 1988 In: Advances in environmental sciences and technology. New York: Wiley and sons: 25

  • Ochi T, Otsuka T, Oshawa M (1988) Glutathione and metallothioneins as cellular defense against cadmium toxicity in cultured Chinese hamster cell. Chem Biol Interact 65:1–14

    Article  PubMed  CAS  Google Scholar 

  • Onkelinx C, Becker J, Sunderman FW Jr (1973) Compartmental analysis of the metabolism of Ni (II) in rats and rabbits. Res Commun Chem Pathol Pharmacol 6:663

    PubMed  CAS  Google Scholar 

  • Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70(1):158–169

    PubMed  CAS  Google Scholar 

  • Sagun KC, Carcamo JM, Golde DW (2005) Vitamin C enters mitochondria via facilitative glucose transporter 1(glut-1) and confers mitochondrial protection against oxidative injury. FASEB J 19(2):1657–1667

    Article  CAS  Google Scholar 

  • Salnikow K, Donald SP, Bruick RK, Zhitkovich A, Phang JM, Kasprzak KS (2004) Depletion of intracellular ascorbate by the carcinogenic metal nickel and cobalt results in the induction of hypoxic stress. J Biol Chem 279:40337–40344

    Article  PubMed  CAS  Google Scholar 

  • Sarkar S, Yadav P, Trivedi R, Bansal AK, Bhatnagar D (1995) Cadmium-induced lipid peroxidation and the status of antioxidant system in rat tissues. J Trace Elem Med Biol 9:144–149

    PubMed  CAS  Google Scholar 

  • Sarkar S, Yadav P, Trivedi R, Bansal AK, Bhatnagar D (1998) Lipid peroxidative damage on cadmium exposure and alterations in antioxidant system in rat erythrocytes: a study with relation to time. Bimetals 11: 153–155

    Article  CAS  Google Scholar 

  • Scansetti G, Maina G, Botta GC et al (1998) Exposure to cobalt and nickel in the hard metal production industry. Int Arch Occup Environ Health 71(1):60–63

    Article  PubMed  CAS  Google Scholar 

  • Shaikh ZA, Vu TT, Zaman K (1999) Oxidative stress as a mechanism of chronic cadmium induced hepatotoxicity and renal toxicity and protection by antioxidants. Toxicol Appl Pharmacol 15:256–263

    Article  Google Scholar 

  • Shi H, Noguchi N, Niki E (1999) Comparative study on dynamics of antioxidative action of ∞-tocopheryl hydroquinone, ubiquinol and α-tocopherol against lipid peroxidation. Free Radic Biol Med 27:334-346

    Article  PubMed  CAS  Google Scholar 

  • Shivarajashankara YM, Shivashankara AR, Gopalkrishna PB, Hanumanth Rao S (2001) Effect of fluoride intoxications on lipid peroxidation and antioxidant systems in rats. Floride 34(2):108–113

    CAS  Google Scholar 

  • Steel RGD, Torrie JH. 1960 In: Principles and procedures of statistics. New York: McGraw-Hill: 99–131

  • Tkeshelashvili LK, Tsakadze KZ, Khulissairio V (1989) Effect of some nickel compounds on red blood cell characteristics. Biol Trace Elem Res 21:337–342

    PubMed  CAS  Google Scholar 

  • US NIOSH/OSHA.1985 Pocket guide to chemical hazards, Wahington DC, US National Institute of Occupational Safety and Health, Occupational Safety and Health Administration (Publication No. 85.114)

  • USAT. 1990 Nickel. In: Installation, restoration programme toxicology guide. Marry G. Armstrong Aerospace Medical Research Laboratory. OH: Wright Patterson AFB: Vol 5

  • Vest RS, Gonzales LL,Permann SA, Spencer E,Hamsen LD,Judd AM, Bell JD (2004) Divalent cations increase lipid order in erythrocytes and susceptibility to secretary phospholipids A2. Biophys J 86:2251–2260

    Article  PubMed  CAS  Google Scholar 

  • Whanger PD (1973) Effects of dietary nickel on enzyme activities and mineral content in rats. Toxicol Appl Pharmacol 26:323–331

    Article  Google Scholar 

Download references

Acknowledgements

The authors greatly acknowledge Director, Defence Institute of Physiology and Allied Sciences, Delhi, DRDO, Ministry of Defence, Government of India for providing financial assistance (Ref.No.TC/260/TASK-91 (KKD)/DIPAS/2004 Dated 07/06/2004) to carry out this work. The authors also acknowledge the Dr. B.S. Patil, Dean of Al-Ameen Medical College, Bijapur for his kind cooperation and Mr. Saleem Pathan, Animal House Incharge and Mr. Abid Jahagirdar, Computer operator, Al-Ameen Medical College, Bijapur, Karnataka, India for their technical assistance in this study.

Author information

Authors and Affiliations

  1. Department of Physiology, Al-Ameen Medical College, Bijapur, 586108, Karnataka, India

    Kusal K. Das, Amrita Das Gupta & Salim A. Dhundasi

  2. Department of Pathology, Al-Ameen Medical College, Bijapur, 586108, Karnataka, India

    Ashok M. Patil

  3. Department of Engineering Chemistry, B.L.D.E.A’s. Dr. P.G. Halakatti College of Engineering and Technology, Bijapur, 586103, Karnataka, India

    Swastika N. Das

  4. Department of Biochemistry, Sri B.M. Patil Medical College, Bijapur, 586103, Karnataka, India

    Jeevan G. Ambekar

Authors
  1. Kusal K. Das
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Amrita Das Gupta
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Salim A. Dhundasi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ashok M. Patil
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Swastika N. Das
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jeevan G. Ambekar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kusal K. Das.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Das, K.K., Gupta, A.D., Dhundasi, S.A. et al. Protective role of l-ascorbic acid on antioxidant defense system in erythrocytes of albino rats exposed to nickel sulfate. Biometals 20, 177–184 (2007). https://doi.org/10.1007/s10534-006-9025-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10534-006-9025-z

Keywords

Search

Navigation