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Species-specific Cd-stress Response in the White Rot Basidiomycetes Abortiporus biennis and Cerrena unicolor

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Abstract

The effect of cadmium (Cd) on fungal growth, Cd bioaccumulation and biosorption, and on the formation of potential heavy metal response indicators such as thiols, oxalate, and laccase was investigated in the white rot fungi Cerrena unicolor andAbortiporus biennis. Only the highest Cd concentration employed (200 μM) inhibited growth of C. unicolor, whereas already lower Cd concentrations caused decreasing mycelia dry weights in A. biennis. Cd biosorption onto the mycelial surface was the predominant Cd sequestration mechanism in C. unicolor. Surface-bound and bioaccumulated Cd concentrations were essentially in the same range in A. biennis, leading to considerably higher intracellular Cd concentrations in A. biennis than in C. unicolor. Oxalate and laccase were produced by both of the fungal strains and their extracellular levels were elevated upon Cd exposure. Oxalate concentrations and laccase titres were considerably higher in C. unicolor than in A. biennis. Both fungi responded to increasing Cd concentrations by increasing intracellular amounts of thiol compounds (cysteine, γ-glutamylcysteine, glutathione in both its reduced and oxidized form) but Cd application increased the amounts of thiols to a higher extend in A. biennis. Taken together, these species-specific responses towards Cd suggest that C. unicolor possesses a more efficient system than A. biennis to keep intracellular Cd concentrations low.

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Authors and Affiliations

  1. Department of Biochemistry, Maria Curie-Skłodowska University, Skłodowska Place 3, 20-031, Lublin, Poland

    Anna Jarosz-Wilkołazka & Marcin Grąz

  2. Division of Ecological and Plant Biochemistry, Department of Biochemistry/Biotechnology, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Strasse 3, D-06120, Halle/Saale, Germany

    Bianca Braha, Sieglinde Menge & Gerd-Joachim Krauss

  3. Department of Groundwater Microbiology, UFZ Centre for Environmental Research Leipzig-Halle, Theodor-Lieser-Str. 4, D-06120, Halle, Germany

    Dietmar Schlosser

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  1. Anna Jarosz-Wilkołazka
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  2. Marcin Grąz
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  3. Bianca Braha
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  4. Sieglinde Menge
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  5. Dietmar Schlosser
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  6. Gerd-Joachim Krauss
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Correspondence to Anna Jarosz-Wilkołazka.

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Jarosz-Wilkołazka, A., Grąz, M., Braha, B. et al. Species-specific Cd-stress Response in the White Rot Basidiomycetes Abortiporus biennis and Cerrena unicolor. Biometals 19, 39–49 (2006). https://doi.org/10.1007/s10534-005-4599-4

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