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Sources of dissolved organic matter (DOM) in a Rocky Mountain stream using chemical fractionation and stable isotopes

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Abstract

Dissolved organic matter (DOM) is an important vehicle for the movement of nutrients from terrestrial to aquatic systems. To investigate how the source and composition of aquatic DOM change in both space and time, we used chemical, spectroscopic, and isotopic analyses to characterize DOM in a headwater catchment in the Colorado Front Range. Streamwater samples for DOM analyses were collected from 2 sites, a lightly vegetated alpine site and a forested, subalpine site, in the North Boulder Creek catchment during the snowmelt runoff season (May–September). Concentrations of dissolved organic carbon (DOC) peaked on the ascending limb of the snowmelt hydrograph at both the alpine (2.6 mg C l−1) and the subalpine sites (7.0 mg C l−1) and decreased sharply on the descending limb of the hydrograph. Fractionation of DOM into operationally defined humic and non-humic components showed that the fulvic acid content of DOC decreased through the season at both sites and that spectroscopic (fluorescence and ultraviolet) properties of the humic DOM fraction shifted in a manner consistent with an increase in the proportion of humic DOM derived from instream sources as compared to terrestrial catchment sources. Humic and non-humic fractions of DOM isolated near peak flow in June and during low flows in September showed a seasonal enrichment in 15N and 13C as well as a seasonal decrease in the ratio of aromatic to aliphatic carbon, both of which were correlated with a decrease in the C:N ratio of the DOM fractions. These results suggest that seasonal shifts in the isotopic and chemical characteristics of DOM are a result of changes in catchment sources of DOM. In particular, it appears that DOM production in alpine lakes is an important contributor to the streamwater DOM load during late season low flows, especially in the alpine reach of the catchment. Our results further suggest that stable isotopes of C and N are useful tools, particularly when combined with ancillary data such as elemental analyses and catchment discharge, for evaluating sources and transformations of DOM at the catchment scale.

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References

  • G.A. Aiken D.M. McKnight K.A. Thorn E.M. Thurman (1992) ArticleTitleIsolation of hydrophilic organic acids from water using nonionic macroporous resins Org. Geochem. 18 567–573

    Google Scholar 

  • Aiken G.A., McKnight D.M., Harnish R. and Wershaw R. 1996. Geochemistry of aquatic humic aubstances in the Lake Fryxell Basin, Antarctica. Biogeochemistry 34: 157–188.

    Google Scholar 

  • J. Baron D.M. McKnight A.S. Denning (1991) ArticleTitleSources of dissolved and particulate organic material in Loch Vale WatershedRocky Mountain National Park, Colorado, USA Biogeochemistry 15 89–109

    Google Scholar 

  • J.S. Baron D.S. Ojima E.A. Holland W.J. Parton (1994) ArticleTitleAnalysis of nitrogen saturation potential in Rocky Mountain tundra and forest: implications for aquatic systems Biogeochemistry 27 61–82

    Google Scholar 

  • E.W. Boyer G.M. Hornberger K.E. Bencala D.M. McKnight (1997) ArticleTitleResponse characteristics of DOC flushing in an alpine catchment Hydrol. Process 11 1635–1647

    Google Scholar 

  • P.D. Brooks D.M. McKnight K.E. Bencala (1999) ArticleTitleThe relationship between soil heterotrophic activity, soil dissolved organic carbon (DOC) lechateand catchment-scale DOC export in headwater catchments Water Resour. Res 35 1895–1902

    Google Scholar 

  • I. Buffam J.N. Galloway L.K. Blum K.J. McGlathery (2001) ArticleTitleA stormflow/baseflow comparison of dissolved organic matter concentrations and bioavailability in an Appalachian stream Biogeochemistry 53 269–306

    Google Scholar 

  • N. Caine (1989) ArticleTitleHydrograph separation in a small alpine basin based on inorganic solute concentrations J. Hydrol 112 89–101

    Google Scholar 

  • N. Caine (1995) ArticleTitleTemporal trends in the quality of streamwater in an alpine environment: Green Lakes Valley, Colorado Front Range, USA Geografiker Annaler 77A 207–220

    Google Scholar 

  • Y.P. Chin G.R. Aiken E. O’Loughlin (1994) ArticleTitleMolecular weight polydispersivity, and spectroscopic properties of aquatic humic substances Environ. Sci. Technol 28 1853–1858

    Google Scholar 

  • C.C. Cleveland J.C. Neff A.R. Townsend E. Hood (2004) ArticleTitleComposition, dynamics, and fate of leached dissolved organic material in terrestrial ecosystems: results from a decomposition experiment Ecosystems 7 275–285

    Google Scholar 

  • J.J. Cole M.L. Pace S.R. Carpenter J.F. Kitchell (2000) ArticleTitlePersistence of net heterotrophy in lakes during nutrient addition and food web manipulations Limnol. Oceanogr 45 IssueID8 1718–1730

    Google Scholar 

  • Croue J.P., DeBroux J.F., Aiken G.R., Leenheer J.A. and Amy G.L. 1999. Natural organic matter: structural characteristics and reactive properties. In: Singer P. (ed.), Formation and Control of Disinfection By-Products in Drinking Water. American Waterworks, Association, pp. 65–92.

  • J.F. Elder N.B. Rybicki V. Carter V. Weintraub (2000) ArticleTitleSources and yields of dissolved carbon in northern Wisconsin stream catchments with differing amounts of peatland Wetalnds 20 113–125

    Google Scholar 

  • E. Gardner (2003) Effects of nutrient enrichment on phytoplankton in an alpine lake Colorado USA Department of Civil and Environmental Engineering University of Colorado Boulder

    Google Scholar 

  • C.T. Garten L.W. Cooper W.M. Post P.J. Hanson (2000) ArticleTitleClimate controls on forest soil C isotope ratios in the southern Appalachian mountains Ecology 81 1108–1119

    Google Scholar 

  • E.W. Hood M.W. Williams N. Caine (2002) ArticleTitleYields of dissolved organic C, N and P from three high-elevation catchments, Colorado Front Range, USA WaterAirSoil Pollut.: Focus 2 165–180

    Google Scholar 

  • E.W. Hood D.M. McKnight M.W. Williams (2003a) ArticleTitleSources and chemical quality of dissolved organic carbon (DOC) across an alpine/subalpine ecotoneGreen Lakes Valley, Colorado Front Range, USA Water Resour. Res 39 IssueID7 1188

    Google Scholar 

  • E.W. Hood M.W. Williams N. Caine (2003b) ArticleTitleOrganic and inorganic N export across an alpine–subalpine ecotone Green Lakes Valley, Colorado Ecosystems 6 31–45

    Google Scholar 

  • G.M. Hornberger K.E. Bencala D.M. McKnight (1994) ArticleTitleHydrological controls on dissolved organic carbon during snowmelt in the Snake River near Montezuma Colorado Biogeochemistry 25 147–165

    Google Scholar 

  • J. Hruska H. Laudon C.E. Johnson S. Kohler K. Bishop (2001) ArticleTitleAcid/base character of organic acids in a boreal stream during snowmelt Water Resour. Res 37 1043–1056 Occurrence Handle10.1029/2000WR900290

    Article  Google Scholar 

  • E.W.D. Huffman H.A. Stuber (1985) Analytical methodology for elemental analysis of humic substances G.R. Aiken D.M. McKnight R.L. Wershaw P. MacCarthy (Eds) Humic Substances in Soil, Sedimentand Water John Wiley and Sons New York 433–456

    Google Scholar 

  • A.P. Hunt J.D. Parry J. Hamilton-Taylor (2000) ArticleTitleFurther evidence of elemental composition as an indicator of the bioavailability of humic substances to bacteria Limnol. Oceanogr 45 237–241

    Google Scholar 

  • S.S. Kaushal W.M. Lewis (2003) ArticleTitlePatterns in the chemical fractionation of organic nitrogen in Rocky Mountain streams Ecosystems 6 483–492

    Google Scholar 

  • C. Kendall (1998) Tracing nitrogen cources and cycling in catchments C. Kendall J.J. McDonnell (Eds) Isotope Tracers in Catchment Hydrology Elsevier Amsterdam 519–576

    Google Scholar 

  • C. Kendall S.R. Silva V.J. Kelly (2001) ArticleTitleCarbon and nitrogen isotopic compositions of particulate organic matter in four large river systems across the United States Hydrol. Process. 15 1301–1346

    Google Scholar 

  • L. Klapper D.M. McKnight J.R. Fulton E.L. Blunt-Harris K.P. Nevin D.R. Lovely P.G. Hatcher (2002) ArticleTitleFulvic acid oxidation state detection using fluorescence spectroscopy Environ. Sci. Technol. 36 3170–3175 Occurrence Handle12141500

    PubMed  Google Scholar 

  • W.M. Lewis M.C. Grant (1980) ArticleTitleRelationships between snow cover and winter losses of dissolved substances from a mountain watershed Arct. Alp. Res 12 11–17

    Google Scholar 

  • Liu F., Williams M.W. and Caine N. 2004. Source waters and flow paths in an alpine catchment, Colorado Front Range, USA. Water Resour. Res. 40(9): W09401. doi 10.1029/2004WR003076.

  • R.L. Malcolm (1990) ArticleTitleThe uniqueness of humic substances in each soil, stream and marine environments Anal. Chim. Acta 232 19–30

    Google Scholar 

  • D.M. McKnight G.R. Aiken R.L. Smith (1991) ArticleTitleAquatic fulvic acids in microbially based ecosystems: results from two desert lakes in Antarctica Limnol. Oceanogr 36 IssueID5 998–1006

    Google Scholar 

  • D.M. McKnight K.E. Bencala G.W. Zellweger G.R. Aiken G.L. Feder K.A. Thorn (1992) ArticleTitleSorption of dissolved organic carbon by hydrous aluminum and iron oxides occurring at the confluence of Deer Creek with the Sanke River Summit County, Colorado Environ. Sci. Technol 26 1388–1396

    Google Scholar 

  • D.M. McKnight E.D. Andrews S.A. Spaulding G.R. Aiken (1994) ArticleTitleAquatic fulvic acids in algal-rich antarctic ponds Limnol. Oceanogr 39 1972–1979

    Google Scholar 

  • D.M. McKnight R. Harnish R.L. Wershaw J.S. Baron S. Schiff (1997) ArticleTitleChemical characteristics of particulate colloidal and dissolved organic material in Loch Vale Watershed Rocky Mountain Nationa Park Biogeochemistry 36 99–124

    Google Scholar 

  • D.M. McKnight E.W. Boyer P.K. Westerhoff P.T. Doran T. Kulbe D.T. Anderson (2001) ArticleTitleSpectrofluorometric characterization of dissolved organic matter for indication of precursor organic material and aromaticity Limnol. Oceanogr. 46 38–48

    Google Scholar 

  • D.M. McKnight G.M. Hornberger K.E. Bencala E.W. Boyer (2002) ArticleTitleIn-stream sorption of fulvic acid in an acidic stream: a stream-scale transport experiment Water Resour. Res 38 IssueID1 1005

    Google Scholar 

  • D.M. McKnight E. Hood L. Klapper (2003) Trace organic moieties of dissolved organic material in natural waters S.E.G. Findlay R.L. Sinsabaugh (Eds) Aquatic Ecosystems: Interactivity of Dissolved Organic Matter Academic Press Amsterdam 71–93

    Google Scholar 

  • N. Mladenov (2004) Evaluating the effects of hydrologic change in the Okavango Delta of Botswana: an analysis of aquatic organic matter transport and ecosystem economics Department of Civil and Environmental Engineering University of Colorado Boulder

    Google Scholar 

  • M.A. Moran R.E. Hodson (1990) ArticleTitleBacterial production on humic and nonhumic components on dissolved organic carbon Limnol. Oceanogr. 35 1744–1756

    Google Scholar 

  • D.P. Morris H. Zagarese C.E. Williamson E.G. Balseiro B.R. Hargreaves B. Modenutti R. Moeller C. Queimalinos (1995) ArticleTitleThe attenuation of solar UV radiation in lakes and the role of dissolved organic carbon Limnol. Oceanogr. 40 1381–1391

    Google Scholar 

  • Mulder J. and Clarke N. 2000. Production and transport of organic solutes: effects of natural climate variation. EU PROTOS project Final Report.

  • S.M. Palmer D. Hope M.F. Billet J.J.C. Dawson C. Bryant (2001) ArticleTitleSources of organic and inorganic carbon in a headwater stream: evidence from carbon isotope studies Biogeochemistry 52 321–338

    Google Scholar 

  • B.J. Peterson R.W. Howarth (1987) ArticleTitleSulfur carbon, and nitrogen isotopes used to trace organic matter flow in the salt-march estuaries of Sapelo Island, Georgia Limnol. Oceanogr 32 1195–1213

    Google Scholar 

  • P.A. Raymond J.E. Bauer (2001) ArticleTitleUse of 14C and 13C natural abundances for evaluating riverine estuarine and coastal DOC and POC sources and cycling: a review and synthesis Org. Geochem 32 469–485

    Google Scholar 

  • S.L. Schiff R. Aravena S.E. Trumbore P.J. Dillon (1990) ArticleTitleDissolved organic carbon cycling in forested watersheds: a carbon isotope approach Water Res. Resour 26 2949–2957

    Google Scholar 

  • S.L. Schiff R. Aravena S.E. Trumbore M.J. Hinton R. Elgood P.J. Dillon (1997) ArticleTitleExport of DOC from forested catchments on the Precambrian Shield of Central Ontario: clues from 13C and 14C Biogeochemistry 36 43–65

    Google Scholar 

  • N.M. Scully D.R.S. Lean (1994) ArticleTitleThe attenuation of UV radiation in temperate lakes Arch. Hydrobiol 43 135–144

    Google Scholar 

  • J.O. Sickman A. Leydecker J.M. Melack (2001) ArticleTitleNitrogen mass balances and abiotic controls on N retention and yield in high-elevation catchments of the Sierra NevadaCaliforniaUnited States Water Resour. Res. 37 IssueID5 1445–1461

    Google Scholar 

  • J.K. Sueker J.N. Ryan C. Kendall R.D. Jarrett (2000) ArticleTitleDetermination of hydrologic pathways during snowmelt for alpine–subalpine basins, Rocky Mountain National Park, Colorado Water Resour. Res. 36 IssueID1 63–76

    Google Scholar 

  • E.M. Thurman (1985) Organic Geochemistry of Natural Waters Martinus Nijhoff/Dr W. Junk Dordrecht, Netherlands 497

    Google Scholar 

  • Thurman E.M. and Malcolm R.L. 1981. Preperative isolation of aquatic humic aubstances. Enviorn. Sci. Technol. 15: 463–466.

    Google Scholar 

  • Waters S.B. McKnight D.M., Wolfe A.P., Vinebrooke R., Sunderman S. and O’Brien M. 2004. Algal and biogeochemical responses to environmental change in an alpine lake. J. Phycol. in press.

  • J.L. Weishaar G.R. Aiken E. Depaz B. Bergamaschi M. Fram R. Fujii (2003) ArticleTitleEvaluation of specific ultra-violet absorbance as an indicator of the chemical composition and reactivity of dissolved organic carbon Environ. Sci. Technol 37 4702–4708 Occurrence Handle14594381

    PubMed  Google Scholar 

  • R.L. Wershaw (1985) Application of nuclear magnetic resonance spectroscopy for determining functionality in humic substances G.R. Aiken D.M. McKnight R.L. Wershaw P. MacCarthy (Eds) Humic Substances in Soil, Sediment and Water John Wiley & Sons New York 561–582

    Google Scholar 

  • R.G. Wetzel (1995) ArticleTitleDeath detritus, and energy flow in aquatic ecosystems Freshwat. Biol 33 83–89

    Google Scholar 

  • M.W. Williams J.S. Baron N. Caine R. Sommerfeld R. Sanford SuffixJr. (1996a) ArticleTitleNitrogen saturation in the Rocky Mountains Environ. Sci. Technol 30 640–646

    Google Scholar 

  • M.W. Williams M. Losleben N. Caine D. Greenland (1996b) ArticleTitleChanges in climate and hydrochemical responses in a high-elevation catchment Rocky Mountains Limnol. Oceanogr 41 939–946

    Google Scholar 

  • M.H. Williams E. Hood N. Caine (2001) ArticleTitleRole of organic nitrogen in the nitrogen cycle of a high-elevation catchment Colorado Front Range. Water Resour. Res 37 2569–2581

    Google Scholar 

  • A.P. Wolfe J.S. Baron R.J. Cornett (2001) ArticleTitleAnthropogenic nitrogen deposition induces rapid ecological changes in alpine lakes of the Colorado Front Range (USA) J. Paleolimnol 25 1–7

    Google Scholar 

  • A.P. Wolfe S.S. Kaushal J.R. Fulton D.M. McKnight (2002) ArticleTitleSpectrofluorescence of sediment humic substances and historical changes of lacustrine organic matter provenance in response to atmospheric nutrient enrichment Environ. Sci. Technol. 36 3217–3223 Occurrence Handle12188343

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Natural Sciences, University of Alaska Southeast, 99801, Juneau, AK, USA

    Eran Hood

  2. Department of Geography, Institute of Arctic and Alpine Research, University of Colorado, 80309-0450, Boulder, CO, USA

    Mark W. Williams

  3. Departmnet of Civil Engineering, Institute of Arctic and Alpine Research, University of Colorado, 880309-0450, Boulder, CO, USA

    Diane M. McKnight

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  1. Eran Hood
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Correspondence to Eran Hood.

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Hood, E., Williams, M. & McKnight, D. Sources of dissolved organic matter (DOM) in a Rocky Mountain stream using chemical fractionation and stable isotopes. Biogeochemistry 74, 231–255 (2005). https://doi.org/10.1007/s10533-004-4322-5

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