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Characteristics and metabolic pathway of acetamiprid biodegradation by Fusarium sp. strain CS-3 isolated from soil

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Abstract

An acetamiprid-degrading fungus was isolated from contaminated soil and identified as Fusarium sp. strain CS-3 based on physiological, biochemical, and molecular analyses. Strain CS-3 exploited 50 mg/L as the sole carbon source in liquid culture, removing 98% in 96 h. Strain CS-3 retained its acetamiprid degradation abilities over a wide range of pH (5.0–8.0) and temperature (20–42 °C). HPLC–MS analysis showed that N′-[(6-chloropyridin-3-yl)methyl]-N-methylacetamide, 2-chloro-5-hydroxymethylpyridine, and 6-chloronicotinic acid were identified as the most predominant metabolites, forming the basis for a newly described acetamiprid degradation pathway. Strain CS-3 efficiently degraded 99.6% of 50 mg/kg acetamiprid in soil, indicating potential for soil remediation.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31700092, No. 21727818, No. 21390200, No. 21706125, No. 21706124), the Jiangsu Province Natural Science Foundation for Youths (No. BK20170997, No. BK20170993), The Key Science and Technology Project of Jiangsu Province (No. BE2016389), The Project of State Key Laboratory of Materials Oriented Chemical Engineering (KL17-09), and The Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture (XTE1834).

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Authors and Affiliations

  1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu Road No. 30, Nanjing, 211800, People’s Republic of China

    Zhoukun Shi, Weiliang Dong, Fengxue Xin, Jiawei Liu, Xinhai Zhou, Fanli Xu, Ziyao Lv, Jiangfeng Ma, Yan Fang & Min Jiang

  2. Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing, 211800, People’s Republic of China

    Weiliang Dong, Fengxue Xin, Jiangfeng Ma, Wenming Zhang, Yan Fang & Min Jiang

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  1. Zhoukun Shi
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  10. Yan Fang
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  11. Min Jiang
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Correspondence to Weiliang Dong or Min Jiang.

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Shi, Z., Dong, W., Xin, F. et al. Characteristics and metabolic pathway of acetamiprid biodegradation by Fusarium sp. strain CS-3 isolated from soil. Biodegradation 29, 593–603 (2018). https://doi.org/10.1007/s10532-018-9855-8

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  • DOI: https://doi.org/10.1007/s10532-018-9855-8

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