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Isolation and characterization of Pseudomonas sp. DX7 capable of degrading sulfadoxine

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Abstract

Given that the intensive application of sulfonamides in aquaculture, animal husbandry and malaria treatment has lead to an increase in sulfonamide discharge into the environment, there is an increasing need to find a way to remediate sulfonamide-contaminated sites. The bacterial strain DX7 was isolated from a marine environment and is capable of degrading sulfadoxine. DX7 was identified as a Pseudomonas sp. based on 16S rRNA gene sequencing. Approximately 30% of sulfadoxine was degraded after Pseudomonas sp. DX7 was inoculated into mineral salt plus tryptone media containing 10 mg l−1 sulfadoxine for 2 days. The degradation efficiency under different environmental conditions was characterized using HPLC. The optimal temperature and pH for sulfadoxine biodegradation were around 30°C and 6.0, respectively. The optimal concentrations of sulfadoxine and tryptone for sulfadoxine biodegradation were determined to be approximately 30 mg l−1 and between 2.0 and 8.0 g l−1, respectively. Cytotoxicity analysis indicated that the metabolites of sulfadoxine generated by Pseudomonas sp. DX7 showed significantly reduced cytotoxicity to Hela cells. These results suggest that Pseudomonas sp. DX7 is a new bacterial resource for degrading sulfadoxine and indicate the potential of the isolated strain in the bioremediation of sulfadoxine-contaminated environments.

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Acknowledgments

This work was financially supported by Innovation Projects of the Chinese Academy of Sciences grant KZCX2-EW-206, the National Natural Science Foundation of China (NSFC) grant 20975089, the Doctoral Foundation of Shandong Province grant BS2011SW056, the Department of Science and Technology of Yantai City of China grant 2010235, and the 100 Talents Program of the Chinese Academy of Sciences.

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Authors and Affiliations

  1. Key Laboratory of Coastal Environmental Processes, Chinese Academy of Sciences, 17 Chunhui Road, Yantai, 264003, China

    Weiwei Zhang, Zongliang Niu, Kun Yin, Ping Liu & Lingxin Chen

  2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Dongxue Xu

  3. Graduate University of the Chinese Academy of Sciences, Beijing, 100049, China

    Dongxue Xu & Kun Yin

  4. Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, Chinese Academy of Sciences, 17 Chunhui Road, Yantai, 264003, China

    Weiwei Zhang, Zongliang Niu, Kun Yin, Ping Liu & Lingxin Chen

  5. Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, 17 Chunhui Road, Yantai, 264003, China

    Weiwei Zhang, Zongliang Niu, Kun Yin, Ping Liu & Lingxin Chen

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  1. Weiwei Zhang
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  2. Dongxue Xu
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  3. Zongliang Niu
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  4. Kun Yin
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  5. Ping Liu
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  6. Lingxin Chen
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Corresponding author

Correspondence to Lingxin Chen.

Additional information

Weiwei Zhang and Dongxue Xu contributed equally to this study.

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Zhang, W., Xu, D., Niu, Z. et al. Isolation and characterization of Pseudomonas sp. DX7 capable of degrading sulfadoxine. Biodegradation 23, 431–439 (2012). https://doi.org/10.1007/s10532-011-9522-9

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  • DOI: https://doi.org/10.1007/s10532-011-9522-9

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