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Degradation of phenanthrene by the endophytic fungus Ceratobasidum stevensii found in Bischofia polycarpa

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Abstract

Some strains of white rot fungi, non-lignolytic fungi and litter-decomposing basidiomycetes have been recognized as PAH degraders. The purpose of our research was to enlarge the scope of PAH-degrading fungi and explore the huge endophytic microorganism resource for bioremediation of PAHs. In this study, phenanthrene was used as a model PAHs compound. Nine strains of endophytic fungi isolated from four kinds of plant from Eupharbiaceae were screened for degradation of phenanthrene. The endophytic fungus Ceratobasidum stevensii (strain B6) isolated from Bischofia polycarpam showed high degradation efficiency and was selected for further studies. Into the fungal culture, 100 mg l−1 phenanthrene was added, and after 10 days of incubation, about 89.51% of the phenanthrene was removed by strain B6. Extracellular ligninolytic enzyme activities of strain B6 were tested. The results showed that manganese peroxidase [MnP] was the predominant ligninolytic enzyme and that its production was greatly induced by the presence of phenanthrene. To confirm the involvement of MnP in phenanthrene degradation, promotion and inhibition studies on MnP in different concentration level of Mn2+ and NaN3 were performed. Additionally, fungal mycelium-free and resuspended experiments were carried out. The results showed no apparent correlation between MnP activity and phenanthrene degradation. The mycelium and fresh medium were the crucial factors affecting the degradation of phenanthrene. To date, this is the first report on PAH degradation by Ceratobasidum stevensii. This study suggests that endophytic fungi might be a novel and important resource for microorganisms that have PAH-degrading capabilities.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (No. 30770073, 30500066). The authors express their great thanks to reviewers and the editorial staff for their time and attention.

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Authors and Affiliations

  1. Jiangsu key Laboratory for Biodiversity and Biotechnology, Life Science College of Nanjing Normal University, 210046, Nanjing, China

    Chuan-chao Dai, Yu-ting Zhao, Yan Chen & Hui Xie

  2. Jiangsu Vocational and Technical College of Finance & Economics, 223302, Huai´an, China

    Lin-shuang Tian

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  1. Chuan-chao Dai
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Correspondence to Chuan-chao Dai.

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Dai, Cc., Tian, Ls., Zhao, Yt. et al. Degradation of phenanthrene by the endophytic fungus Ceratobasidum stevensii found in Bischofia polycarpa . Biodegradation 21, 245–255 (2010). https://doi.org/10.1007/s10532-009-9297-4

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