Abstract
Understanding the effect of heavy metals and wood preservatives on the growth of wood-rot fungi native to a certain region may improve reliability in determining the effectiveness of antifungal products, particularly when dealing with new formulations. In this investigation, strains of copper-tolerant wood-rot fungi native to south-central Chile were evaluated against two preservatives: commercial chromated copper arsenate type C (CCA-C) and a new formulation with boron and silicon (BS). Thirteen native strains, mainly white-rot fungi, were selected for their high growth rates in solid medium containing 3 mM of copper. A short-term test was then carried out, consisting of adding cellulose disks impregnated with different concentrations of preservatives to solid culture media inoculated with selected copper tolerant strains. There was a great variability in interspecific and intraspecific responses to the presence of copper and preservatives in culture media. Among the native and commercial strains evaluated, the white-rot fungi Trametes versicolor 38 and mainly Ganoderma australe 100 were notable for their tolerance to all the CCA-C and BS concentrations. The brown-rot fungus Wolfiporia cocos, used as reference strain, showed a high tolerance to CCA-C, but not to BS preservative. T. versicolor 38 and G. australe 100 were selected for subsequent studies on preserved wood degradation.
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References
Alleman B, Logan B, Gilbertson R (1993) A rapid method to screen fungi for resistance to toxic chemicals. Biodegradation 4:125–129. doi:10.1007/BF00702329
American Wood Preservers’ Association (AWPA) (1999) Standard method P5-97. In: Book of Standards. AWPA, Granbury, TX
American Wood Preservers’ Association (AWPA) (2004) Standard method E10-01. In: Book of Standards. AWPA, Granbury, TX
Archer K, Nicholas D, Schultz T (1995) Screening of wood preservatives: comparison of the soil-block, agar-block, and agar-plate tests. Forest Prod J 45:86–89
Baldrian P (2003) Interactions of heavy metals with white-rot fungi. Enzyme Microb Technol 32:78–91. doi:10.1016/S0141-0229(02)00245-4
Bernicchia A (2005) Fungi Europaei, vol. 10. Polyporaceae s.I. Ed. Candusso, Italy
Borokhov O, Rothenburger S (2000) Rapid dye decolorization method for screening potential wood preservatives. Appl Environ Microbiol 66:5457–5459. doi:10.1128/AEM.66.12.5457-5459.2000
Clausen C, Green FIII, Woodward B, Evans J, De Groot R (2000) Correlation between oxalic acid production and copper tolerance in Wolfiporia cocos. Int Biodeter Biodegr 46:69–76. doi:10.1016/S0964-8305(00)00044-5
Collet O (1992) Comparative tolerance of the brown-rot fungus Antrodia vallantii (DC.:Fr.) Ryv. isolates to copper. Holzforschung 46:293–298
Chapman W, Berch S, Ballard T (1990) In vitro growth of ectomycorrhizal fungi on dilute agar. Mycologia 82:526–527. doi:10.2307/3760029
De Groot R, Woodward B (1999) Using copper-tolerant fungi to biodegrade wood treated with copper-based preservatives. Int Biodeter Biodegr 44:17–27. doi:10.1016/S0964-8305(99)00047-5
Escobar N (2005) Impregnante BS. Aliado ecológico para la madera. Lignum Chil 83:76–83
Green FIII, Clausen C (2005) Copper tolerance of brown-rot fungi: oxalic acid production in southern pine treated with arsenic-free preservatives. Int Biodeter Biodegr 56:75–79. doi:10.1016/j.ibiod.2005.04.003
Guillén Y, Machuca A (2008) The effect of copper on the growth of wood-rotting fungi and a blue-stain fungus. World J Microbiol Biotechnol 24:31–37. doi:10.1007/s11274-007-9434-3
Humar M, Pohleven F (2005) Influence of a nitrogen supplement on the growth of wood decay fungi and decay of wood. Int Biodeter Biodegr 56:34–39. doi:10.1016/j.ibiod.2005.03.008
Humar M, Bokan M, Sentjurc M, Amartey S, Kalan P, Pohleven F (2004) Fungal bioremediation of copper, chromium and boron treated wood as studied by electron paramagnetic resonance. Int Biodeter Biodegr 53:25–32. doi:10.1016/j.ibiod.2003.08.001
Humar M, Bucar B, Pohleven F (2006) Brown-rot decay of copper-impregnated wood. Int Biodeter Biodegr 58:9–14. doi:10.1016/j.ibiod.2006.03.003
Humar M, Žlindra D, Pohleven F (2007) Improvement of fungicidal properties and copper fixation of copper–ethanolamine wood preservatives using octanoic acid and boron compounds. Holz Roh Werkst 65:17–21. doi:10.1007/s00107-006-0118-8
Illman B, Highley T (1996) Fungal degradation of wood treated with metal-based preservatives: 1. Fungal tolerance. International Research Group on Wood Preservation, IRG/WP 96-10163, Stockholm, Sweden
Illman B, Yang V (2004) Bioremediation and degradation of CCA-C-treated wood waste. In: Proceedings of environmental impacts of preservative-treated wood. Center Environmental Solutions Gainesville, Florida, USA, p 10
Illman B, Yang V, Ferge L (2000) Bioprocessing preservative-treated waste wood. International Research Group on Wood Preservation, IRG/WP 00-501545, Stockholm, Sweden
Lebow S (2004) Alternatives to chromated copper arsenate for residential construction. Res Pap FPL-RP-618. USDA, For Service, For Prod Lab, Madison WI, 9 p
Monrroy M, Freer J, Baeza J, Rodríguez J (2006) Degradation of tribromophenol by wood-rot fungi and Hamilton system. Electron J Biotechnol 9:253–257. doi:10.2225/vol9-issue3-fulltext-26
Monrroy M, Baeza J, Freer J, Rodríguez J (2007) Degradation of tribromophenol by wood-decaying fungi and the 1, 2-dihydroxybenzene-assisted Fenton reaction. Bioremediat J 11:195–200. doi:10.1080/10889860701710560
Murphy R, Levy J (1983) Production of copper oxalate by some copper tolerant fungi. Transact Br Mycol Soc 81:165–168
Navarrete P (2004) Innovación nacional: La petrificación de la madera. Bit-La Revista Técnica de la Construcción (Chile) 37:52–55
Pohleven F, Humar M, Amartey S, Benedik J (2002) Tolerance of wood decay fungi to commercial copper based wood preservatives. International Research Group on Wood Preservation, IRG/WP/3029, Stockholm, Sweden
Przewloka S (2004) Comparison of rapid decay testing methodologies for the screening of new wood preservatives. Forest Wood Products Research, Development Corporation, Report No. PG04.5015, p 81
Rajchenberg M (2006) Los poliporos (Basidiomycetes) de los bosques andino patágonicos de Argentina. Biblioteca Mycologica; J Cramer, Germany
Ryvarden L (1987) New and noteworthy polypores from Tropical America. Mycotaxon 28:525–541
Ryvarden L (1991) Genera of Polypores. Nomenclature and taxonomy. Fungiflora, Norway
Schultz T, Nicholas D, Henry W, Pittman C, Wipf D, Goodell B (2005) Review of laboratory and outdoor exposure efficacy results of organic biocide: antioxidant combinations, an initial economic analysis and discussion of a proposed mechanism. Wood Fiber Sci 37:175–184
Tham L, Matsuhashi S, Kume T (1999) Responses of Ganoderma lucidum to heavy metals. Mycoscience 40:209–213. doi:10.1007/BF02464301
Woodward B, De Groot R (1999) Tolerance of Wolfiporia cocos isolates to copper in agar media. Forest Prod J 49:87–94
Wright J, Deschamps J (1972) Basidiomicetos xilófagos de los bosques andinopatagónicos. Revista de Investigaciones Agropecuarias (INTA). Argentina. Patol Vegetal 9:1–195
Acknowledgments
The authors wish to thank the valuable technical support of Forest Engineer Daniel Chávez M. We also wish to thank A. Franzani and O. Inostroza for donating the CCA-C and BS preservatives.
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Guillén, Y., Navias, D. & Machuca, Á. Tolerance to wood preservatives by copper-tolerant wood-rot fungi native to south-central Chile. Biodegradation 20, 135–142 (2009). https://doi.org/10.1007/s10532-008-9207-1
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DOI: https://doi.org/10.1007/s10532-008-9207-1