Abstract
Mushroom production on coffee pulp as substrate generates an intense black residual liquid, which requires suitable treatment. In the present study, Pleurotus ostreatus growth in wastewater from mushroom farm was evaluated as a potential biological treatment process for decolourisation as well as to obtain biomass (liquid inoculum). Culture medium components affecting mycelial growth were determined, evaluating colour removal. Laccase activity was monitored during the process. P. ostreatus was able to grow in non diluted WCP. Highest biomass yield was obtained when glucose (10 g/l) was added. The addition of this carbon source was necessary for efficient decolourisation. Agitation of the culture improved biodegradation of WCP as well as fungal biomass production. Laccase and manganese-independent peroxidase activities were detected during fungal treatment of the WCP by P. ostreatus CCEBI 3024. The laccase enzyme showed good correlation with colour loss. Both wastewater colour and pollution load (as chemical oxygen demand) decreased more than 50% after 10 days of culture. Phenols were reduced by 92%.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
American Public Health Association (1998) Standard methods for the examination of water and wastewater, 20th edn. Washington, DC, USA, p 1124
Ander P, Marzullo L (1997) Sugar oxidoreductases and veratryl alcohol oxidase as related to lignin degradation. J Biotechnol 53:115–131
Aust SD, Benson J (1993) The fungus among us-use of white rot fungi to biodegrade environmental pollutants. Environ Health Perspect 101:232–233
Bello R, Sánchez JE (1997) Anaerobic filter treatment of wastewater from mushroom cultivation on coffee pulp. World J Microbiol Biotechnol 13:51–55
Bermúdez RC, García N, Gross P, Serrano M (2001) Cultivation of Pleurotus on agricultural substrates in Cuba. Micología Aplicada Internacional 13(1):25–29
Camarero S, Sarkar S, Ruiz-Dueñas FJ, Martínez MJ, Martínez AT (1999) Description of a versatile peroxidase involved in natural degradation of lignin that has both Mn-peroxidase and lignin-peroxidase substrate binding sites. J Biol Chem 274:10324–10330
Carbajo JM, Junca H, Terron MC, Gonzalez T, Yague S, Zapico E, Gonzalez AE (2002) Tannic acid induces transcription of laccase gene cglccl in the white-rot fungus Coriolopsis gallica. Can J Microbiol 48(12):1041–1047
Cohen R, Persky L, Hadar Y (2002) Biotechnological applications and potential of wood-degrading mushrooms of the genus Pleurotus. Appl Microbiol Biotechnol 58:582–594
Coulibaly L, Gourene G, Agathos (2003) Utilization of fungi for biotreatment of raw wastewaters: review. Afr J Biotechnol 2(12):620–630
Dahiya J, Singh D, Nigam P (2001) Decolourisation of synthetic and spentwash melanoidins using the white-rot fungus Phanerochaete chrysosporium JAG-40. Bioresour Technol 78(1):95–98
Dias A, Bezerra R, Pereira N (2004) Activity and elution profile of laccase during biological decolorization and dephenolization of olive mill wastewater. Bioresour Technol 92:7–13
Dubois M, Guilles K, Hamilton J, Roberts P, Smith F (1956) Colourimetric determination of sugars and related substances. Anal Chem 28:350–356
Field J, Lettinga G (1987) The effect of oxidative coloration on the methanogenic toxicity and anaerobic biodegradability of phenols. Biol Waste 29:161–179
Fountoulajkis M, Dokianakis M, Kornaros M, Aggelis G, Lyberatos G (2002) Removal of phenolics in olive mill wastewaters using the white-rot fungus Pleurotus ostreatus. Water Res 36:4735–4744
Green TR (1977) Significance of glucose oxidase in lignin degradation. Nature 268(5615):78–80
Guillén F, Martínez AT, Martínez MJ (1992) Substrate specificity and properties of the aryl-alcohol oxidase from the ligninolytic fungus Pleurotus eryngii. Eur J Biochem 209:603–611
Guillén G, Márquez F, Sánchez J (1998) Producción de biomasa y enzimas ligninolíticas por Pleurotus ostreatus en cultivo sumergido. Rev Iberoam Micol 15:302–306
Heinfling A, Martínez MJ, Martínez AT, Bergbauer M, Szewzyk U (1998) Purification and characterization of peroxidases from the dye-decolorizing fungus Bjerkandera adusta. FEMS Microbiol Lett 165:43–50
Kim B, Ryu S, Shin K (1996) Effect of culture parameters on the decolorization of Remazol brilliant blue R by Pleurotus ostreatus. J Microbiol 34:101–104
Kissi M, Mountandar M, Asshobei O, Gargiulo E, Palmieri G, Giardina P, Sannia G (2001) Roles of two white-rot basidiomycete fungi in decolorisation and detoxification of olive mill waste water. Appl Microbiol Biotechnol 57:221–226
Leonowicz A, Cho N, Luterek J, Wilkolazka A, Wotjas M, Matus A, Hofritchter M, Wesenberg D, Rogalski J (2001) Fungal laccase: properties and activity on lignin. J Basic Microbiol 41:185–227
Leonowicz A, Rogalski J, Jaszek M, Luterek J, Wasilewska M, Malarczyk E, Ginalska G, Fink-Boots M, Cho N (1999) Cooperation of fungal laccase and glucose 1-oxidase in transformation of Bjorkman lignin and some phenolic compounds. Holzforschung 53:376–380
Maestro D, Borja R, Martin A, Fiesta JA, Mendoza J (1991) Biodegradación de los compuestos fenólicos presentes en el alpechín. Fasc 42:271–276
Marques de Souza C, Tychanowicz G, Farani D, Peralta R (2004) Production of laccase isoforms by Pleurotus pulmonarius in response to presence of phenolic and aromatic compounds. J Basic Microbiol 44:129–136
Martínez AT, Camarero S, Guillén F, Gutiérrez A, Muñoz C, Varela M, Martínez E, Barrosa J, Ruel K, Pelayo M (1994) Progress in biopulping of non woody materials: chemical, enzymatic and ultrastructural aspects of wheat–straw delignification with ligninolytic fungi from the genus Pleurotus. FEMS Microbiol Rev 13:265–284
Martínez MJ, Ruiz-Dueñas FJ, Guillén F, Martínez AT (1996) Purification and catalytic properties of two manganese–peroxidase isoenzymes from Pleurotus eryngii. Eur J Biochem 237:424–432
Martínez-Carrera D (2000) Mushroom biotechnology in tropical America. Int J Mushroom Sci 3:9–20
Miller G (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem 31:426–428
Nieto-López C, Sánchez-Vazquez J (1997) Mycelial growth of Pleurotus and Auricularia in agroindustrial effluents. Micología Neotropical Aplicada 10:47–56
Palmieri G, Giardina P, Bianco C, Fontanella B, Sannia G (2000) Copper induction of laccase isoenzymes in the ligninolytic fungus Pleurotus ostreatus. Appl Environ Microbiol 66:920–924
Ramírez J, Clifford M (2000) Coffee pulp polyphenols: an overview. In: Sera T, Soccol CR, Pandey A, Roussos S (eds) Coffee biotechnology and quality. Kluwer, Dordrecht, pp 471–488
Riva S (2006) Laccases: blue enzymes for green chemistry. Trends Biotechnol 24(5):219–226
Rodríguez S, Fernández M, Bermúdez RC, Morris H (2003) Tratamiento de efluentes industriales coloreados con Pleurotus spp. Rev Iberoam Micol 20:164–168
Scalbert A (1991) Antimicrobial properties of tannins. Phytochemistry 30:875–883
Tien M, Kirk TK (1984) Lignin-degrading enzyme from Phanerochaete chyrsosporium: purification, characterization and catalytic properties of a unique H2O2-requiring oxygenase. PNAS-Biol Sci 81(8):2280–2284
Yesilada O, Asma D, Cing S (2003) Decolorization of textile dyes by fungal pellets. Process Biochem 38:933–938
Yesilada O, Sik S, Sam M (1998) Biodegradation of olive oil mill wastewater by Coriolus versicolur and Funalia trogi: effects of agitation, initial COD concentration, inoculum size and immobilization. World J Microbiol Biotechnol 14:37–42
Zhang F, Knapp J, Tapley K (1999) Decolourisation of cotton bleaching effluent with wood rotting fungus. Water Res 33(4):919–928
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Rodríguez Pérez, S., García Oduardo, N., Bermúdez Savón, R.C. et al. Decolourisation of mushroom farm wastewater by Pleurotus ostreatus . Biodegradation 19, 519–526 (2008). https://doi.org/10.1007/s10532-007-9157-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10532-007-9157-z