Abstract
Objectives
To determine furfural biotransformation capabilities of Acinetobacter baylyi ADP1 and Acinetobacter schindleri ACE.
Results
Acinetobacter baylyi ADP1 and A. schindleri ACE could not use furfural as sole carbon source but when acetate was used as substrate, ADP1 and ACE biotransformed 1 g furfural/l in 5 and 9 h, respectively. In both cases, the product of this biotransformation was difurfuryl-ether as shown by FT-IR and 1H and 13C NMR spectroscopy. The presence of furfural decreased the specific growth rate in acetate by 27% in ADP1 and 53% in ACE. For both strains, the MIC of furfural was 1.25 g/l. Nonetheless, ADP1 biotransformed 2 g furfural/l at a rate of 1 g/l/h in the stationary phase of growth. A transcriptional analysis of possible dehydrogenases involved in this biotransformation, identified that the areB and frmA genes were highly overexpressed after the exposure of ADP1 to furfural. The products of these genes are a benzyl-alcohol dehydrogenase and an alcohol dehydrogenase.
Conclusions
Acinetobacter baylyi ADP1 is a candidate for the biological detoxification of furfural, a fermentation inhibitor present in lignocellulosic hydrolysates, with the possible direct involvement of the AreB and FrmA enzymes in the process.
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Acknowledgements
We thank technical support by Dr. Gabriel Vigueras Ramírez and Ernesto García García (Universidad Autónoma Metropolitana, México).
Funding
This study was funded by the Consejo Nacional de Ciencia y Tecnología (CONACyT) [CB-2012–01/183 813], and Programa Especial de Apoyo a la Investigación Básica 2019 Rectoría General Universidad Autónoma Metropolitana, to Sigala JC.
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Arteaga, J.E., Cerros, K., Rivera-Becerril, E. et al. Furfural biotransformation in Acinetobacter baylyi ADP1 and Acinetobacter schindleri ACE. Biotechnol Lett 43, 1043–1050 (2021). https://doi.org/10.1007/s10529-021-03094-1
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DOI: https://doi.org/10.1007/s10529-021-03094-1