Abstract
Objective
This study is to explore the exact roles of extracellular vesicle (EVs) miRNAs from brain microvascular pericytes in the pathogenesis of hypertension.
Results
Forty-eight significantly differentially expressed miRNAs were identified, of which 17 were found to be upregulated and 31 were found to be downregulated in brain microvascular pericytes of spontaneous hypertensive rats, compared with that of normotension Wistar Kyoto rats. The GO enrichment analysis verified that the target genes were enriched in signaling pathways and molecular functions, such as metal ion binding, nucleotide binding and ATP binding. The KEGG analysis indicated that the target genes were enriched in Linoleic acid, alpha-linolenic acid and sphingolipid metabolism pathways.
Conclusions
Several EV derived miRNAs, such as miR-21-5p, let-7c-5p and let-7a-5p, were found to be abnormally expressed in brain microvascular pericytes obtained from spontaneous hypertensive rats, compared with that of normotension Wistar Kyoto rats. The results of our research provide more insights into the functional link between brain microvascular pericytes and the pathogenesis of hypertension.
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Abbreviations
- UTR:
-
Untranslated regions
- EVs:
-
Extracellular vesicles
- SCI:
-
Spinal cord injury
- TEM:
-
Transmission electron microscopy
- NTA:
-
Nanoparticle tracking analysis
- KEGG:
-
Kyoto Encyclopedia of Genes and Genomes
- SHR:
-
Spontaneous hypertensive rats
- WKY:
-
Wistar Kyoto rats
- GO:
-
Gene ontology
- BP:
-
Biology process
- CC:
-
Cellular component
- MF:
-
Molecular function
- SLC7A1:
-
Solute carrier family 7 member 1
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Acknowledgements
This study was supported by the innovation fund of the Chinese Academy of Medical Sciences and Peking Union Medical College (Grant Nos. 3332014006 and 3332015123), the CAMS Initiative for Innovative Medicine (CAMS-I2M) (Grant No. 2016-I2M-3-006) and National Natural Science Foundation of China (Grant No. 81801433).
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Wu, Q., Yuan, X., Li, B. et al. Differential miRNA expression analysis of extracellular vesicles from brain microvascular pericytes in spontaneous hypertensive rats. Biotechnol Lett 42, 389–401 (2020). https://doi.org/10.1007/s10529-019-02788-x
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DOI: https://doi.org/10.1007/s10529-019-02788-x