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MiRNA-125a-5p attenuates blood–spinal cord barrier permeability under hypoxia in vitro

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Abstract

Disruption of the blood–spinal cord barrier (BSCB) results in secondary injury and apoptosis of neurons, leading to permanent neurological dysfunction after spinal cord injury. In this study, we evaluate the role of miRNA-125a-5p in the BSCB under hypoxia. The miRNA-125a-5p mimics group showed lower horseradish peroxidase (HRP) permeability and endothelial cell death rates compared with the transfection control group. By contrast, the miRNA-125a-5p inhibitor group demonstrated higher HRP permeability and endothelial cell death rates than the transfection control group. The expressions of ZO-1, occludin, VE-cadherin and their mRNA significantly increased in miRNA-125a-5p-overexpressing cells. By contrast, a remarkable reduction in ZO-1, occludin, and VE-cadherin expression and their mRNA were observed in miRNA-125a-5p-inhibited cells. MiRNA-125a-5p appears to reduce the permeability of the BSCB by up regulating the expression of ZO-1, occludin, and VE-cadherin and their mRNA, and against hypoxia-induced apoptosis of spinal cord microvascular endothelial cells. Taken together, our results clearly indicate that miRNA-125a-5p plays an important role in protecting the functions of the BSCB under hypoxia.

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Acknowledgements

This work is supported by National Natural Science Foundation of China (Grant Nos. 81471853, 81501659 and 81801906) and Natural Science Foundation of Shandong Province of China (Grant No. ZR2018PH024).

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Authors and Affiliations

  1. Department of Orthopedics, Jining No. 1 People’s Hospital, Jining, 272000, China

    Jian Wang, Zhikui Nie & Kai Gao

  2. Department of Emergency, Jining No. 1 People’s Hospital, Jining, 272000, China

    Huanhua Zhao

  3. Department of Orthopedics, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, 121000, China

    Yang Cao

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  1. Jian Wang
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  2. Zhikui Nie
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  3. Huanhua Zhao
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  4. Kai Gao
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  5. Yang Cao
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Correspondence to Yang Cao.

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Wang, J., Nie, Z., Zhao, H. et al. MiRNA-125a-5p attenuates blood–spinal cord barrier permeability under hypoxia in vitro. Biotechnol Lett 42, 25–34 (2020). https://doi.org/10.1007/s10529-019-02753-8

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  • DOI: https://doi.org/10.1007/s10529-019-02753-8

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