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High-content screening of Aspergillus niger with both increased production and high secretion rate of glucose oxidase

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Abstract

Objectives

To develop a rapid, dual-parameter, plate-based screening process to improve production and secretion rate of glucose oxidase simultaneously in Aspergillus niger.

Results

A morphology engineering based on CaCO3 was implemented, where the yield of GOD by A. niger was increased by up to 50%. Analysis of extracellular GOD activity was achieved in 96-well plates. There was a close negative correlation between the total GOD activity and its residual glucose of the fermentation broth. Based on this, a rapid, plate-based, qualitative analysis method of the total GOD activity was developed. Compared with the conventional analysis method using o-dianisidine, a correlation coefficient of −0.92 by statistical analysis was obtained.

Conclusion

Using this dual-parameter screening method, we acquired a strain with GOD activity of 3126 U l−1, which was 146% higher than the original strain. Its secretion rate of GOD was 83, 32% higher than the original strain.

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Acknowledgements

This work was financially supported by National Major Scientific and Technological Special Project (2012YQ15008709) and National Key Research and Development Program (2017YFB0309302).

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O. Box 329, Shanghai, 200237, People’s Republic of China

    Xudong Zhu, Jingchun Sun & Ju Chu

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  1. Xudong Zhu
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  2. Jingchun Sun
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  3. Ju Chu
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Correspondence to Ju Chu.

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Zhu, X., Sun, J. & Chu, J. High-content screening of Aspergillus niger with both increased production and high secretion rate of glucose oxidase. Biotechnol Lett 40, 103–110 (2018). https://doi.org/10.1007/s10529-017-2442-y

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  • DOI: https://doi.org/10.1007/s10529-017-2442-y

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