Abstract
Objectives
To investigate gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8, a potential high-ethanol producer, in response to various stresses during high-temperature ethanol fermentation using sweet sorghum juice (SSJ) under optimal conditions.
Results
The maximal ethanol concentration obtained by S. cerevisiae KKU-VN8 using SSJ at 40 °C was 66.6 g/l, with a productivity of 1.39 g/l/h and a theoretical ethanol yield of 81%. Quantitative RT-PCR assays were performed to investigate the gene expression profiles of S. cerevisiae KKU-VN8. Differential expression of genes encoding heat-shock proteins (HSP82, HSP104, SSA4), genes involved in trehalose metabolism (TPS1, TPS2, NTH1) and genes involved the glycolytic pathway (ADH1, ADH2, CDC19) at various time points during fermentation was observed. The expression levels of HSP82, HSP104, SSA4, ADH1 and CDC19 were significantly higher than those of the controls (10.2-, 4-, 8-, 8.9- and 5.9-fold higher, respectively). In contrast, the expression levels of TPS1, TPS2, NTH1 and ADH2 were approx. 2-fold less than those of the controls.
Conclusions
The highly expressed genes encoding heat-shock proteins, HSP82 and SSA4, potentially play an important role in helping S. cerevisiae KKU-VN8 cope with various stresses that occur during high-temperature fermentation, leading to higher ethanol production efficiency.
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Acknowledgements
This research was financially supported by the National Research University Project of Thailand through the Biofuel Research Cluster of Khon Kaen University, Thailand (NRU 543042 and NRU-KKU-Ph.D 54310) and the Energy Conservation Fund of Energy Policy and Planning office (EPPO), Ministry of Energy, Thailand. We are grateful to the Fermentation Research Center for Value Added Agricultural Products (FerVAAP), the Department of Biotechnology, Faculty of Technology, Khon Kaen University and Molecular biology research laboratory, Faculty of Dentistry, Khon Kaen University for supporting the equipment and facilities used in this research.
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Supplementary Table 1—Gene-specific primers.
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Techaparin, A., Thanonkeo, P. & Klanrit, P. Gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8 during high-temperature ethanol fermentation using sweet sorghum juice. Biotechnol Lett 39, 1521–1527 (2017). https://doi.org/10.1007/s10529-017-2398-y
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DOI: https://doi.org/10.1007/s10529-017-2398-y