Abstract
Objectives
To identify whether lncRNAs (long non-coding RNA) participate in the regulation of cisplatin-resistant induced autophagy in endometrial cancer cells.
Results
Autophagy activity was significantly boosted in cisplatin-resistant Ishikawa cells, a human endometrial cancer cell line, compared with that in parental Ishikawa cells. After analyzing the overall long noncoding RNA (lncRNA) profiling, a meaningful lncRNA, HOTAIR, was identified. It was down-regulated simultaneously in cisplatin-resistant Ishikawa cells and parental Ishikawa cells treated with cisplatin. RNA interference of HOTAIR reduced the proliferation of cisplatin-resistant Ishikawa cells and enhanced the autophagy activity of cisplatin-resistant Ishikawa cells with or without cisplatin treatment, in addition, beclin-1, multidrug resistance (MDR), and P-glycoprotein (P-gp) were mediated by lncRNA HOTAIR.
Conclusions
It is clear that lncRNAs, specifically HOTAIR, can regulate the cisplatin-resistance ability of human endometrial cancer cells through the regulation of autophagy by influencing Beclin-1, MDR, and P-gp expression.
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Change history
30 December 2023
This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1007/s10529-023-03458-9
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Acknowledgements
This work was supported by the funds from Scientific and Technologic Innovation Action of Shanghai Municipal Science and Technology Commission (15401971800 and 15401902700), Shanghai Municipal Health and Family Planning Commission (ZY3-JSFC-2-2010, 20134173 and 20134090), Outstanding Leaders Training Program of Pudong Health Bureau of Shanghai (PWR12015-05), the State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources (ME2016012) and the Talents Training Program of Seventh People’s Hospital of Shanghai University of TCM (XX2017-01).
Supporting information
Supplementary Fig. 1—Identification of cisplatin-resistant Ishikawa human endometrial cancer cell line.
Supplementary Fig. 2—LncRNA profiling in Ishikawa and Ishikawa CP in absence and presence of CP.
Supplementary Table 1—List of lncRNAs tested.
Supplementary Table 2—Primers sequences of lncRNAs.
Supplementary Table 3—Expression of lncRNAs in Ishikawa and Ishikawa CP cells in absence and presence of CP.
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Sun, MY., Zhu, JY., Zhang, CY. et al. RETRACTED ARTICLE: Autophagy regulated by lncRNA HOTAIR contributes to the cisplatin-induced resistance in endometrial cancer cells. Biotechnol Lett 39, 1477–1484 (2017). https://doi.org/10.1007/s10529-017-2392-4
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DOI: https://doi.org/10.1007/s10529-017-2392-4