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Diabetic nephropathy: serum miR-9 confers a poor prognosis in and is associated with level changes of vascular endothelial growth factor and pigment epithelium-derived factor

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Abstract

Objective

To investigate the relationship between the serum level of miR-9 and the progression of diabetic nephropathy (DN) and related molecular mechanisms.

Results

Thirty-five healthy subjects and 140 DN patients were divided into five groups: control, DN I–II, DN III, DN IV and DN V. Serum level of miR-9 was measured by real-time qPCR. Serum levels of vascular endothelial growth factor (VEGF), pigment epithelium-derived factor (PEDF) lipids, fasting glucose, insulin, hemoglobin A1c (HBA1c), creatinine, fibrinogen and insulin resistance (HOMA-IR) were also measured. The results show that the levels of miR-9, PEDF and VEGF are increased with DN progression (P < 0.05). miR-9, VEGF and PEDF are independent risk factors of DN (R2 = 0.430). Spearman rank correlation analysis showed that miR-9 level is positively related to the levels of VEGF, PEDF, cholesterol, triglyceride, fasting glucose, fasting insulin, HBA1c, creatinine, fibrinogen and HOMA-IR (P < 0.05).

Conclusions

Serum miR-9 is a potential marker for conferring a poor prognosis in DN and associated with the levels of VEGF, PEDF and biochemical indices.

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Authors and Affiliations

  1. Department of Endocrinology, First Center Hospital of Baoding City, 320 North Greatwall Street, Baoding, 071000, China

    Yanxin Xiao, Shuqin Guo, Yunliang Zhang, Zhiying Bian, Liyan Jia, Yanyun Hu, Jie Chen, Chao Yin, Ning Li, Dongxun Zhang, Xincui Zhao & Jun Wang

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  1. Yanxin Xiao
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  2. Shuqin Guo
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  3. Yunliang Zhang
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  4. Zhiying Bian
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  5. Liyan Jia
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  6. Yanyun Hu
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  7. Jie Chen
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  8. Chao Yin
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  9. Ning Li
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  10. Dongxun Zhang
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  11. Xincui Zhao
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  12. Jun Wang
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Corresponding author

Correspondence to Jun Wang.

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Xiao, Y., Guo, S., Zhang, Y. et al. Diabetic nephropathy: serum miR-9 confers a poor prognosis in and is associated with level changes of vascular endothelial growth factor and pigment epithelium-derived factor. Biotechnol Lett 39, 1583–1590 (2017). https://doi.org/10.1007/s10529-017-2390-6

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  • DOI: https://doi.org/10.1007/s10529-017-2390-6

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