Abstract
Objectives
To investigate the biological functions of microRNA-144-3p with respect to proliferation and apoptosis of human salivary adenoid carcinoma cell lines via mTOR.
Results
After transfection of microRNA-144-3p agomir, cell viability assays confirmed that the salivary adenoid carcinoma cell (SACC) proliferation was inhibited and apoptosis was induced. Dual luciferase reporter assay validated that the mammalian target of rapamycin (mTOR) was a direct target of miR-144-3p. Western blot, immunofluorescent analysis and a xenograft mouse model of adenoid cystic carcinoma indicated that miR-144-3p was a tumor suppressor and repressed mTOR expression and signaling in SACCs.
Conclusions
MicroRNA-144-3p inhibits proliferation and induces apoptosis of human salivary adenoid carcinoma cells by downregulating mTOR expression in vitro and in vivo.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China 81400545 for C.Z and 81170991 for H.H.
Supporting information
Supplementary Fig. 1—MTOR is a direct target of miR-144-3p.
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The authors declare no conflict of interest.
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Fangyi Huo and Chen Zhang contributed equally to this work.
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Huo, F., Zhang, C., He, H. et al. MicroRNA-144-3p inhibits proliferation and induces apoptosis of human salivary adenoid carcinoma cells via targeting of mTOR. Biotechnol Lett 38, 409–416 (2016). https://doi.org/10.1007/s10529-015-2007-x
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DOI: https://doi.org/10.1007/s10529-015-2007-x