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Rapid extraction and purification of lumbrokinase from Lumbricus rubellus using a hollow fiber membrane and size exclusion chromatography

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Abstract

Objectives

To develop a purification process using hollow fiber membrane separation combined with size exclusion chromatography for the extraction of lumbrokinase from earthworms (Lumbricus rubellus).

Results

To extract the protein, the earthworms were first homogenized for 10 min, to produce ultrafine particles. Polyether sulfone hollow fiber membranes with MW cut offs of 50 and 6 kDa were used for initial purification of the crude extract. Further purification was carried out on a Sephadex G-75 column, and yielded three fractions of high purity protein. One of these fractions showed fibrinolytic activity.

Conclusions

Three samples of high purity protein were obtained and one protein (LK1) showed strong fibrinolytic activity. The method has higher purification efficiency in comparison with existing methods.

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Acknowledgments

This work was supported by funding from the National Natural Science Foundation of China (Grant No. 51403104), Natural Science Foundation of Jiangsu Province (Grant No. BK20141465), and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Authors and Affiliations

  1. School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210029, China

    Tingming Fu, Fengyun Yang, Huaming Zhu, Huaxu Zhu & Liwei Guo

  2. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing, 210029, China

    Tingming Fu

Authors
  1. Tingming Fu
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  2. Fengyun Yang
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  3. Huaming Zhu
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  4. Huaxu Zhu
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  5. Liwei Guo
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Correspondence to Tingming Fu.

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Fu, T., Yang, F., Zhu, H. et al. Rapid extraction and purification of lumbrokinase from Lumbricus rubellus using a hollow fiber membrane and size exclusion chromatography. Biotechnol Lett 38, 251–258 (2016). https://doi.org/10.1007/s10529-015-1979-x

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  • DOI: https://doi.org/10.1007/s10529-015-1979-x

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