Abstract
Objectives
To exploit the archaeal shikimic acid (SA) synthesis pathway toenhance SA production in Corynebacterium glutamicum.
Results
Genetic cassettes were constructed that encoded a hybrid route for SA synthesis, which were composed of the archaeal 6-deoxy-5-ketofructose 1-phosphate pathway and the bacterial SA pathway. Corynebacterium glutamicum strains that expressed the hybrid route increased SA production by 57 %. A recombinant strain of C. glutamicum that simultaneously overexpressed the hybrid route and its native SA pathway produced 4.7 gSA/l, while C. glutamicum with only the native SA pathway produced 3.7 gSA/l.
Conclusions
A new hybrid route for SA production was successfully constructed, that effectively increased SA production in C. glutamicum.
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Acknowledgments
This work was supported by 973 Project from Ministry of Science and Technology (No. 2012CB7211-04).
Supporting information
Supplementary Table 1 Bacterial strains, plasmids, and oligonucleotides used in this study.
Supplementary Fig. 1 Expression of archaeal genes in C. glutamicum RES167ΔaroK/pDKFP-1, RES167ΔaroK/pDKFP-2 and RES167ΔaroK/pDKFP-3.
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Zhang, B., Jiang, CY., Liu, YM. et al. Engineering of a hybrid route to enhance shikimic acid production in Corynebacterium glutamicum . Biotechnol Lett 37, 1861–1868 (2015). https://doi.org/10.1007/s10529-015-1852-y
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DOI: https://doi.org/10.1007/s10529-015-1852-y