Abstract
Water pollution from the release of industrial wastewater is a serious problem for almost every industry. Enzymes from portulaca, Portulaca oleracea cv., have been investigated for their ability to degrade bisphenol A (BPA), one of the well-known estrogenic pollutants. Enzymatic crude extracts from P. oleracea cv. roots were immobilized on aminopropyl-modified glass beads. They maintained BPA metabolic activity over a broad range of pH values and temperatures. The immobilized enzyme was reusable with more than 50 % of its initial activity retained after 12 batch reactions and no loss of activity after storage for 1 month at −30 °C. Thus, the immobilization of extracts from P. oleracea cv. roots is a useful method for removing BPA from industrial wastewater.
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Acknowledgments
This research was supported in part by a Grant-in-Aid for Challenging Exploratory Research (No. 23659064) from Japan Society for the Promotion of Science (JSPS), and a grant from the Japan Chemical Industry Association (JCIA) Long-range Research Initiative (LRI).
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Matsushima, K., Kaneda, H., Harada, K. et al. Immobilization of enzymatic extracts of Portulaca oleracea cv. roots for oxidizing aqueous bisphenol A. Biotechnol Lett 37, 1037–1042 (2015). https://doi.org/10.1007/s10529-014-1761-5
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DOI: https://doi.org/10.1007/s10529-014-1761-5