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In vitro selection of a peptide aptamer that changes fluorescence in response to verotoxin

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Abstract

A peptide aptamer that changes fluorescence upon binding to verotoxin was selected in vitro using ribosome display with a tRNA carrying an environment-sensitive fluorescent probe. The aptamer specifically bound to verotoxin with a dissociation constant (K d) of 3.94 ± 1.6 µM, and the fluorescence decreased by 78 % as the verotoxin concentration was increased. The selected peptide can be used for detection of verotoxin.

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Acknowledgments

The research is financially supported by a Grant-in-Aid for Scientific Research (S) of KAKENHI 22220009 from the Japan Society for the Promotion of Science “and The Cannon Foundation”. We are grateful for the support received from the Brain Science Institute (BSI) Research Resource Center for sequencing, HPLC purification and mass spectrum analysis.

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Authors and Affiliations

  1. Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Yasodha Manandhar, K. C. Tara Bahadur, Wei Wang, Takanori Uzawa, Toshiro Aigaki & Yoshihiro Ito

  2. Graduate School of Biological Science, Tokyo Metropolitan University, 1-1 Minami-Osawa, Tokyo, 192-0397, Japan

    Yasodha Manandhar, Toshiro Aigaki & Yoshihiro Ito

  3. Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Materials Science, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Takanori Uzawa & Yoshihiro Ito

Authors
  1. Yasodha Manandhar
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  2. K. C. Tara Bahadur
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  3. Wei Wang
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  4. Takanori Uzawa
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  5. Toshiro Aigaki
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  6. Yoshihiro Ito
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Correspondence to Yoshihiro Ito.

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Manandhar, Y., Bahadur, K.C.T., Wang, W. et al. In vitro selection of a peptide aptamer that changes fluorescence in response to verotoxin. Biotechnol Lett 37, 619–625 (2015). https://doi.org/10.1007/s10529-014-1719-7

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  • DOI: https://doi.org/10.1007/s10529-014-1719-7

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