Abstract
Bio-electrospraying (BES) is a technique for directly jetting living cells that has significant implications for tissue engineering and regenerative medicine. However, the effect of BES on human adipose-derived stem cells (hASCs) remains unknown. Here, we show that an hASC suspension was successfully electrosprayed via a continuous, stable and linearly directed electrospray at 10 kV and at 3 ml/h. Morphological observations and Trypan Blue and CCK-8 assays revealed that the cells remained viable and proliferated at a rate similar to that of the controls (0 kV). However, at 20 kV, BES became unstable and cell viability was reduced. Moreover, hASCs electrosprayed at 10 kV retained their multilineage potential, successfully differentiating into chondrogenic, osteogenic and neurogenic lineages. Thus, BES does not significantly affect cell morphology, viability or multipotency.
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19 February 2021
A Correction to this paper has been published: https://doi.org/10.1007/s10529-021-03102-4
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Acknowledgments
We thank Mr. Li Rui and Luo Wanlun for their academic support. This work was supported by grants from National Natural Science Foundation of China (81360232), the Key Project of Chinese Ministry of Education (2012156), and the Department of Science and Technology (G20107015, 20103166, LG2012008) and the Department of Education (2009143) of Guizhou Province.
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Supplementary Table 1—Sequences of the primers for the analyzed genes
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Ye, C., He, Z., Lin, Y. et al. Bio-electrospraying is a safe technology for delivering human adipose-derived stem cells. Biotechnol Lett 37, 449–456 (2015). https://doi.org/10.1007/s10529-014-1693-0
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DOI: https://doi.org/10.1007/s10529-014-1693-0