Abstract
The growth rate and maximum biomass of Bacillus coagulans 2–6 were inhibited by lactate; inhibition by sodium lactate was stronger than by calcium lactate. The differences of protein expressions by B. coagulans 2–6 under the lactate stress were determined using two-dimensional electrophoresis coupled with mass spectrometric identification. Under the non-stress condition, calcium lactate stress and sodium lactate stress, the number of detected protein spots was 1,571 ± 117, 1,281 ± 231 and 904 ± 127, respectively. Four proteins with high expression under lactate stress were identified: lactate dehydrogenase, cysteine synthase A, aldo/keto reductase and ribosomal protein L7/L12. These proteins are thus potential targets for the reconstruction of B. coagulans to promote its resistance to lactate stress.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (31200078), the Shandong Provincial Natural Science Foundation, China (ZR2001CQ025), and the CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences (KLIM-201302).
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Wang, X., Qin, J., Wang, L. et al. A comparative proteomic analysis of Bacillus coagulans in response to lactate stress during the production of l-lactic acid. Biotechnol Lett 36, 2545–2549 (2014). https://doi.org/10.1007/s10529-014-1639-6
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DOI: https://doi.org/10.1007/s10529-014-1639-6