Abstract
Biomass and lipid productivities of Isochrysis galbana were optimized using nutrients of molasses (4, 8, 12 g l−1), glucose (4, 8, 12 g l−1), glycerol (4, 8, 12 g l−1) and yeast extract (2 g l−1). Combinations of carbon sources at different ratios were evaluated in which the alga was grown at three different light intensities (50, 100 and 150 μmol m−2 s−1) under the influence of three different photoperiod cycles (12/12, 18/6 and 24/0 h light/dark). A maximum cell density of 8.35 g l−1 with 32 % (w/w) lipid was achieved for mixotrophic growth at 100 μmol m−2 s−1 and 18/6 h light/dark with molasses/glucose (20:80 w/w). Mixotrophic cultivation using molasses, glucose and glycerol was thus effective for the cultivation of I. galbana.
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Babuskin, S., Radhakrishnan, K., Babu, P.A.S. et al. Effect of photoperiod, light intensity and carbon sources on biomass and lipid productivities of Isochrysis galbana . Biotechnol Lett 36, 1653–1660 (2014). https://doi.org/10.1007/s10529-014-1517-2
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DOI: https://doi.org/10.1007/s10529-014-1517-2