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Glyoxalase 1 is up-regulated in hepatocellular carcinoma and is essential for HCC cell proliferation

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Abstract

Glyoxalase 1 (Glo1), belonging to the glyoxalase system, participates in the detoxification of methylglyoxal (MG), a byproduct of glycolysis. Glo1 is associated with the progression of many human malignancies. However, the role of Glo1 in hepatocellular carcinoma (HCC) is unclear. We have discovered that the expression of Glo1 is up-regulated in HCC tissues compared with adjacent non-tumorous tissues, and knockdown of Glo1 expression by RNA interference significantly inhibited the proliferation of human HCC cell lines. Glo1 knockdown resulted in the accumulation of its cytotoxic substrate, MG. Overall, thus Glo1 might be essential for HCC progression and can be designated as a potential therapeutic target for HCC in the future.

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Acknowledgments

We thank Dr. Shoudong Ye for his critical reading of the manuscript. This work was supported by the National Key Sci-Tech Special Project of China (2013ZX10002010).

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 220 Handan Road, Shanghai, 200433, People’s Republic of China

    Xiaohui Hu, Xianmei Yang, Quanze He & Long Yu

  2. Department of Chemistry, Fudan University, 220 Handan Road, Shanghai, 200433, People’s Republic of China

    Qi Chen

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  1. Xiaohui Hu
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  2. Xianmei Yang
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  3. Quanze He
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  4. Qi Chen
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  5. Long Yu
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Correspondence to Long Yu.

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Fig. S1

The effect of Glo1 overexpression on cell proliferation. The proliferation assays were performed in three HCC cell lines: Hep3B (a), SK-HEP-1 (b) and SMMC-7721 (c). Data represent the mean ± SD, n = 5 (TIFF 809 kb)

Fig. S2

The cytotoxic effect of MG on HCC cell lines. MG toxicity assays were performed in three HCC cell lines: Hep3B (a), SK-HEP-1 (b) and SMMC-7721 (c). During a 6-day culture period, the culture mediums were changed everyday with indicated concentrations of MG. Data represent the mean ± SD, n = 5 (TIFF 526 kb)

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Hu, X., Yang, X., He, Q. et al. Glyoxalase 1 is up-regulated in hepatocellular carcinoma and is essential for HCC cell proliferation. Biotechnol Lett 36, 257–263 (2014). https://doi.org/10.1007/s10529-013-1372-6

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  • DOI: https://doi.org/10.1007/s10529-013-1372-6

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