Abstract
Hairy root cultures of Scutellaria lateriflora were established using Agrobacterium rhizogenes A4 and produced acteoside (18.5 mg g−1 dry wt), baicalin (14.5 mg g−1 dry wt) and wogonoside (12 mg g−1 dry wt). Yeast extract (50 μg ml−1) increased acteoside production 1.4-fold and flavone production 1.7-fold after 7 and 14 days of elicitation. Addition of Pectobacterium carotovorum lysate in the stationary phase of the hairy root culture stimulated only the accumulation of wogonin to 30 mg g−1 dry wt. The production of wogonin in hairy roots could be associated with its role as a phytolaexin.
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Wilczańska-Barska, A., Królicka, A., Głód, D. et al. Enhanced accumulation of secondary metabolites in hairy root cultures of Scutellaria lateriflora following elicitation. Biotechnol Lett 34, 1757–1763 (2012). https://doi.org/10.1007/s10529-012-0963-y
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DOI: https://doi.org/10.1007/s10529-012-0963-y