Abstract
The VP6 protein of rotavirus A (RVA) is a target antigen used for diagnostic assays and also for the development of new RVA vaccines. We have compared the expression of VP6 protein in human embryonic kidney (HEK293-T) cells with results obtained using a well-established insect cell-baculovirus system. The recombinant VP6 (rVP6) expressed in HEK293-T cells did not present degradation and also retained the ability to form trimers. In the insect cell-baculovirus system, rVP6 was expressed at higher levels and with protein degradation as well as partial loss of ability to form trimers was observed. Therefore, HEK293-T cells represent a less laborious alternative system than insect cells for expression of rVP6 from human RVA.
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Acknowledgments
This work was sponsored by the Post-graduate Program in Molecular and Cellular Biology (IOC/FIOCRUZ), Foundation for Research Support of Rio de Janeiro Carlos Chagas Filho (FAPERJ) and National Council for Scientific and Technological Development (CNPq).
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da Silva Junior, H.C., da Silva e Mouta Junior, S., de Mendonça, M.C.L. et al. Comparison of two eukaryotic systems for the expression of VP6 protein of rotavirus specie A: transient gene expression in HEK293-T cells and insect cell-baculovirus system. Biotechnol Lett 34, 1623–1627 (2012). https://doi.org/10.1007/s10529-012-0946-z
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DOI: https://doi.org/10.1007/s10529-012-0946-z