Abstract
S HI-R ELATED S EQUENCE (SRS) genes are plant-specific transcription factors containing a zinc-binding RING finger motif, which play a critical role in plant growth and development. We have characterized six SRS genes in Brassica rapa. Overexpression of the SRSs BrSTY1, BrSRS7, and BrLRP1 induced dwarf and compact plants, and significantly decreased primary root elongation and lateral root formation. Additionally, the transgenic plants had upward-curled leaves of narrow widths and with short petioles, and had shorter siliques and low fertility. In stems, hypocotyls, and styles, epidermal cell lengths were also significantly reduced in transgenic plants. RT-PCR analysis of transgenic plants revealed that BrSTY1, BrSRS7, and BrLRP1 regulate expression of several gibberellin (GA)- and auxin-related genes involved in morphogenesis in shoot apical regions. We conclude that BrSTY1, BrSRS7, and BrLRP1 regulate plant growth and development by regulating expression of GA- and auxin-related genes.
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Acknowledgments
This work was carried out with the support of “Research Program for Agricultural Biotechnology (PJ008616)”, National Academy of Agricultural Science, Rural Development Administration, Republic of Korea.J. K. Hong was supported by a 2011 Post-Doctoral Fellowship Program of National Academy of Agricultural Science, Rural Development Administration, Republic of Korea.We thank Mr. Lee Jun Sung in National Academy of Agricultural Science, RDA very much for scanning electron microscope observation.
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Hong, J.K., Kim, J.A., Kim, J.S. et al. Overexpression of Brassica rapa SHI-RELATED SEQUENCE genes suppresses growth and development in Arabidopsis thaliana . Biotechnol Lett 34, 1561–1569 (2012). https://doi.org/10.1007/s10529-012-0929-0
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DOI: https://doi.org/10.1007/s10529-012-0929-0