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An electrochemical method for functionalization of a 316L stainless steel surface being used as a stent in coronary surgery: irreversible immobilization of fibronectin for the enhancement of endothelial cell attachment

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Abstract

An electrochemistry-based method for the formation of functionalized alkanethiol layers on a 316L stainless steel surface was developed. The method was efficient in forming a very stable, irreversibly-attached COOH-terminated (mercaptoundecanoic acid) surface layer. This layer was used as a ‘linker’ to immobilize the extracellular matrix protein fibronectin to the 316L stainless steel surface. Fibronectin was irreversibly attached to the surface and, unlike physisorbed fibronectin, resisted detachment more in aggressive 0.1 M NaOH under sonication. The fibronectin-modified 316L stainless steel surface was more biocompatible towards attachment of endothelial cells than a bare (unmodified) 316L stainless steel surface, yielding a 25% improvement in cell density.

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Acknowledgments

The authors would like to thank the Natural Science and Engineering Research Council of Canada and the Canadian Institutes of Health Research for support of this research, through a Collaborative Health Research Program Grant.

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Author notes

  1. Andreas Bergdahl

    Present address: Department of Exercise Science, Concordia University, Montreal, QC, Canada

Authors and Affiliations

  1. Department of Chemical Engineering, McGill University, University Street, Montreal, QC, 3610, Canada

    Jeffrey Harvey, Hesam Dadafarin & Sasha Omanovic

  2. Department of Anatomy and Cell Biology, McGill University, Montreal, QC, Canada

    Andreas Bergdahl, Li Ling & Elaine C. Davis

Authors
  1. Jeffrey Harvey
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  2. Andreas Bergdahl
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  3. Hesam Dadafarin
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  4. Li Ling
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  5. Elaine C. Davis
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  6. Sasha Omanovic
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Correspondence to Sasha Omanovic.

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Harvey, J., Bergdahl, A., Dadafarin, H. et al. An electrochemical method for functionalization of a 316L stainless steel surface being used as a stent in coronary surgery: irreversible immobilization of fibronectin for the enhancement of endothelial cell attachment. Biotechnol Lett 34, 1159–1165 (2012). https://doi.org/10.1007/s10529-012-0885-8

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  • DOI: https://doi.org/10.1007/s10529-012-0885-8

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